Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PYGB Double Nickase Plasmid (h): sc-406294-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PYGB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PYGB Double Nickase Plasmid (h) and PYGB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PYGB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PYGB Antibody (B-5): sc-518267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PYGB Double Nickase Plasmid (h) | sc-406294-NIC | 20 µg | $410.00 | |||
PYGB Double Nickase Plasmid (h2) | sc-406294-NIC-2 | 20 µg | $410.00 |
PYGB encodes the brain isoform of glycogen phosphorylase, a rate-limiting enzyme that catalyzes glycogen breakdown to glucose-1-phosphate, supporting rapid metabolic adaptation during energy demand. Through control of intracellular glycogen mobilization, PYGB influences glycolytic flux and links nutrient availability to cellular stress responses, redox balance, and ATP homeostasis. PYGB activity is connected to pathways governing carbohydrate metabolism and can modulate cellular resilience under hypoxia or nutrient limitation. Dysregulated glycogen metabolism and altered PYGB expression have been associated with metabolic reprogramming observed in neurological and other tissue contexts, making it a useful target for mechanistic studies of energy regulation.
PYGB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PYGB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PYGB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PYGB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PYGB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.