Pit-1 항체 (5E4) WB, IP와 IF으로 mouse, rat, human, bovine와 porcine origin의 Pit-1을 검출할것을 권장합니다.

Pit-1 항체 (5E4): sc-47762

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Pit-1 항체 (5E4) is rated 4.0 out of 5 by 1.
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데이터 시트

    • Pit-1 항체(5E4) 는 마우스 monoclonal IgG2aκ, 1간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • rat origin의 Pit-1에서 내부에 위치한 30-146 아미노산을 항원으로 사용하였습니다.
    • WB, IP와 IF으로 mouse, rat, human, bovine와 porcine origin의 Pit-1을 검출할것을 권장합니다.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Pit-1 Antibody (5E4) for WB applications. These reagents are now offered in bundles with Pit-1 Antibody (5E4) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using Pit-1 (5E4): sc-47762?

Asked by: Germaine
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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