Phosphatidylethanol
Phosphatidylethanol is a useful chromatographic standard for this assay. In the presence of ethanol, phospholipase D converts phosphatidylcholine to phosphatidylethanol (1,2). This conversion is the basis of a selective and sensitive assay for PLD activity in intact cells (3,4). Protect from light. Hygroscopic. For reviews on PLD mediated signaling see references 5-7.
References
1: M. Kobayashi and J.N. Kanfer J. Neurochem. 1987 48 1597 2: L. Gustavsson and C. Alling Biochem. Biophys. Res. Commun. 1987 142 958 3: M. Liscovitch J. Biol. Chem. 1989 264 1450 4: J.-K. Pai et al. J. Biol. Chem. 1988 263 12472 5: J.H. Exton J. Biol. Chem. 1990 265 1 6: M.M. Billah and J.C. Anthes Biochem. J. 1990 269 281 7: N.T. Thompson et al. Trends Pharmacol. Sci. 1991 12 404
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