Phosphate Buffered Saline: powder for 500 ml of 10X

사용법 :

• Reconstitute powder in ddH20, bringing final volume to 500 ml (10X). Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 5 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.


• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.

pH :

When diluted to a 1X concentration, the pH will be ~7.4.

저장 :

Store at room temperature