Phosphate Buffered Saline: 1 L of 10X A 10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research.

Phosphate Buffered Saline: 1 L of 10X

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Alternate Names: Phosphate Buffered Saline: 1 L of 10X is also known as 10X PBS.
Application Phosphate Buffered Saline: 1 L of 10X is a 10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research.
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Phosphate Buffered Saline: 1 L of 10X is a concentrated buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Phosphate Buffered Saline is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.
Additional sizes, formats, and concentrations available:
Phosphate Buffered Saline: 1 L of 1X (sc-362182)
Phosphate Buffered Saline: 100 ml of 10X (sc-362297)
Phosphate Buffered Saline: 500 ml of 10X (sc-362298)
Phosphate Buffered Saline: 1 L of 20X (sc-362183)
Phosphate Buffered Saline: 5 L of 20X (sc-362299)
Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300)
Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301)
Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302)
Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)


References

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792

2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548

3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994

4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324

5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070

6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.

사용법 :
• Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.



• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
Physical State :
Liquid
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
저장 :
Store at room temperature
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

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PMID: # 35906661  Rao, Y.|Zhu, C.|Suen, HC.|Huang, S.|Liao, J.|Ker, DFE.|Tuan, RS.|Wang, D.| et al. 2022. Stem Cell Res Ther. 13: 380.

PMID: # 36207479  Hu, C.|Wang, B.|Liu, Z.|Chen, Q.|Ishikawa, M.|Lin, H.|Lian, Q.|Li, J.|Li, JV.|Ma, D.|, .| et al. 2022. Cell Biol Toxicol.

PMID: # 33419223  Grotegut, P.|Hoerdemann, PJ.|Reinehr, S.|Gupta, N.|Dick, HB.|Joachim, SC.| et al. 2021. Int J Mol Sci. 22:

PMID: # 28050230  Li, Y. et al. 2016. Genes Cancer. 7: 292-300.

PMID: # 21907703  Enunlu, I. et al. 2011. Biochem. Biophys. Res. Commun. 413: 471-475.

Vera Linn S. Fonnes et al. 2008.

PMID: # 33312217  Res Pharm Sci. 390-400.

PMID: # 32561219  Neuropharmacology. 108182.

PMID: # 29374705  Anticancer Res. 803-809.

PMID: # 32763155  Cell.

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