PACAP Double Nickase Plasmid (h): sc-402741-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PACAP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PACAP Double Nickase Plasmid (h) and PACAP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCYAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PACAP Antibody (F-2): sc-166180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PACAP Double Nickase Plasmid (h) | sc-402741-NIC | 20 µg | $410.00 | |||
PACAP Double Nickase Plasmid (h2) | sc-402741-NIC-2 | 20 µg | $410.00 |
ADCYAP1 encodes pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide that signals primarily through PAC1 (ADCYAP1R1) and the VPAC receptors to stimulate cAMP/PKA, PLC/Ca²⁺, and downstream CREB- and MAPK-regulated transcriptional programs. PACAP modulates neuronal excitability, neuroendocrine secretion, and stress-responsive circuits, and it also influences immune and vascular functions through GPCR-mediated signaling. In cellular models, ADCYAP1 activity intersects with pathways controlling synaptic plasticity, metabolic homeostasis, and neuroinflammatory tone. Dysregulated PACAP signaling has been associated with neurological and neuropsychiatric phenotypes, including altered stress and pain processing and migraine-related mechanisms, making ADCYAP1 a relevant target for mechanistic studies of GPCR-neuropeptide biology.
PACAP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCYAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCYAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCYAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCYAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.