p-Rb 항체 (A-5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Ser 249 and Thr 252 dually-phosphorylated Rb을 검출할것을 권장합니다.

p-Rb 항체 (A-5): sc-377528

p-Rb 항체 (A-5) is rated 3.9 out of 5 by 7.
  • y_2021, m_9, d_25, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_ko_KR, sid_377528, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 139ms
  • REVIEWS, PRODUCT
loading
5
3
4
2
3
1
2
0
1
1

데이터 시트

    • p-Rb 항체(A-5) 는 마우스 monoclonal IgG2bκ, 14간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 안티-p-Rb 항체 (A-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Ser 249 and Thr 252 dually-phosphorylated Rb 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-p-Rb 항체 (A-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • p-Rb (A-5): sc-377528무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for p-Rb Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Rb Antibody (A-5) (see ordering information below).
loading

Could you please provide the concentration of the glue of WB, the membrane transfer conditions, the type of sealing liquid and the sealing time, and the primary concentration?

Asked by: lily 12
Thank you for your question. For the transfer conditions, please reach out to the manufacturer of your transfer equipment. The recommended starting dilution of this primary antibody is 1:50 dilution. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2019-09-06

What application is the blocking peptide sc-377528 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_9, d_25, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_ko_KR, sid_377528, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 120ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 3 out of 5 by from not good for fish protein some unspecific bands showed when do the fish experiment.
Date published: 2017-08-08
Rated 5 out of 5 by from perfect antibody. I applied sc-377528 sample half a month ago to test the changes of p-Rb in my cell after treatment and happy to find a good result.
Date published: 2017-08-08
Rated 4 out of 5 by from very good there was three bands in turbot, primary antibody 1:200, second antibody 1:10000. good enough
Date published: 2017-06-20
Rated 4 out of 5 by from Fairly potent and specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is recommended for Western blotting. There was medium detection of a ~105 kDa target-sized protein in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and only significant cross-reactivity with ~45 kDa protein.
Date published: 2016-12-04
Rated 5 out of 5 by from Good bands in Western Blot acquired Good bands in Western Blot acquired. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear and cytoplasmic Beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human vagina tissue. -SCBT QC
Date published: 2015-03-22
  • y_2021, m_9, d_25, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_ko_KR, sid_377528, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT