p-Rb Antibody (A-5): sc-377528

p-Rb Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that specifically detects dually-phosphorylated forms of p-Rb at Ser 249 and Thr 252 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Rb (A-5) antibody is available in non-conjugated and various conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Retinoblastoma protein (Rb) regulates cell cycle progression at the G1-S transition by acting as a transcriptional repressor. Rb phosphorylation at Ser 249 and Thr 252, mediated by cyclin D-dependent kinases, inactivates Rb and enables cell cycle progression. This modification marks a shift from hypophosphorylated Rb, which actively represses cell cycle genes, to hyperphosphorylated Rb, which permits transcription of genes needed for cell division. Rb phosphorylation dynamics and regulatory mechanisms provide crucial insights into tumorigenesis pathways, especially in pediatric cancers like retinoblastoma where Rb gene mutations disrupt normal cell cycle control.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Rb Antibody (A-5) References:

1. Discovery of a regulatory motif that controls the exposure of specific upstream cyclin-dependent kinase sites that determine both conformation and growth suppressing activity of pRb.  |  Driscoll, B., et al. 1999. J Biol Chem. 274: 9463-71. PMID: 10092628
2. Glutamic acid mutagenesis of retinoblastoma protein phosphorylation sites has diverse effects on function.  |  Barrientes, S., et al. 2000. Oncogene. 19: 562-70. PMID: 10698526
3. Transforming growth factor beta inhibits the phosphorylation of pRB at multiple serine/threonine sites and differentially regulates the formation of pRB family-E2F complexes in human myeloid leukemia cells.  |  Hu, X., et al. 2000. Biochem Biophys Res Commun. 276: 930-9. PMID: 11027571
4. Bacterial Species from Vaginal Microbiota Differently Affect the Production of the E6 and E7 Oncoproteins and of p53 and p-Rb Oncosuppressors in HPV16-Infected Cells.  |  Nicolò, S., et al. 2023. Int J Mol Sci. 24: PMID: 37108333
5. The retinoblastoma protein and cell cycle control.  |  Weinberg, RA. 1995. Cell. 81: 323-30. PMID: 7736585
6. Direct transcriptional repression by pRB and its reversal by specific cyclins.  |  Bremner, R., et al. 1995. Mol Cell Biol. 15: 3256-65. PMID: 7760821
7. Expression of differentially phosphorylated Rb and mutant p53 proteins in myeloid leukemia cell lines.  |  Sen, S., et al. 1993. Leuk Res. 17: 639-47. PMID: 8355507
8. Cancer cell cycles.  |  Sherr, CJ. 1996. Science. 274: 1672-7. PMID: 8939849
9. Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation.  |  Connell-Crowley, L., et al. 1997. Mol Biol Cell. 8: 287-301. PMID: 9190208
10. Rb interacts with histone deacetylase to repress transcription.  |  Luo, RX., et al. 1998. Cell. 92: 463-73. PMID: 9491888

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Rb Antibody (A-5) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Rb Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Rb Antibody (A-5) Specifications

Alternate Product Name: RB1

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Ser 249/Thr 252

Epitope Target: Rb

Epitope Species: human

Product Reactivity/Detection: Ser 249 and Thr 252 dually-phosphorylated Rb

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for p-Rb Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Rb antibody is Rb Antibody (IF8): sc-102.