p-FAK Antibody (2D11): sc-81493

p-FAK Antibody (2D11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tyr 397 phosphorylated FAK in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP). anti-p-FAK antibody (2D11) is available in a non-conjugated form, providing flexibility for various experimental setups. Focal adhesion kinase (FAK) plays a crucial role in cellular signaling and adhesion, acting as a non-receptor tyrosine kinase essential for forming focal adhesions, which link the extracellular matrix to the cytoskeleton. These focal adhesions are vital for processes such as cell migration, proliferation, and survival, as they facilitate communication between cells and their environment. FAK phosphorylation at Tyr 397 serves as a key regulatory site influencing kinase activity and interactions with other signaling molecules. p-FAK levels can vary depending on cellular conditions, such as serum availability and cell cycle status, highlighting FAK′s role in adapting cellular responses to external stimuli. Understanding p-FAK dynamics is essential for elucidating FAK′s involvement in various physiological and pathological processes, including cancer progression and tissue repair.

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p-FAK Antibody (2D11) References:

1. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation.  |  Guan, JL. and Shalloway, D. 1992. Nature. 358: 690-2. PMID: 1379699
2. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets.  |  Lipfert, L., et al. 1992. J Cell Biol. 119: 905-12. PMID: 1385445
3. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin.  |  Hanks, SK., et al. 1992. Proc Natl Acad Sci U S A. 89: 8487-91. PMID: 1528852
4. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.  |  Schaller, MD., et al. 1992. Proc Natl Acad Sci U S A. 89: 5192-6. PMID: 1594631
5. Focal adhesion kinase is negatively regulated by phosphorylation at tyrosine 407.  |  Lim, Y., et al. 2007. J Biol Chem. 282: 10398-404. PMID: 17303567
6. mTORC1/rpS6 and p-FAK-Y407 signaling regulate spermatogenesis: Insights from studies of the adjudin pharmaceutical/toxicant model.  |  Wang, L., et al. 2022. Semin Cell Dev Biol. 121: 53-62. PMID: 33867214
7. Phosphorylated paxillin and phosphorylated FAK constitute subregions within focal adhesions.  |  Bachmann, M., et al. 2022. J Cell Sci. 135: PMID: 35343568
8. Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling.  |  Liu, X., et al. 2023. Int J Oral Sci. 15: 32. PMID: 37532712
9. Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src.  |  Schaller, MD., et al. 1994. Mol Cell Biol. 14: 1680-8. PMID: 7509446
10. Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases.  |  Schlaepfer, DD. and Hunter, T. 1996. Mol Cell Biol. 16: 5623-33. PMID: 8816475

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-FAK Antibody (2D11) has 61 citations in a variety of scientific publications.

Primary antibodies like Anti-p-FAK Antibody (2D11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-FAK Antibody (2D11) Specifications

Alternate Product Name: FAK

Product Clone Name: 2D11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 397

Epitope Target: FAK

Epitope Species: human

Product Reactivity/Detection: Tyr 397 phosphorylated FAK

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 61 citations in various scientific publications

Available Conjugates/Forms for p-FAK Antibody (2D11): N/A

The preferred FAK antibody is FAK Antibody (H-1): sc-1688.