Date published: 2025-9-9

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p-Cofilin 1 항체 (E-5): sc-271921

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데이터 시트
  • p-Cofilin 1 항체(E-5) 는 마우스 monoclonal IgMκ, 13간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 인산화된 Ser 31을 포함하는 짧은 아미노산 서열에 대해 제기됨 Cofilin 1의 human 기원
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Ser 3 phosphorylated Cofilin 1을 검출할것을 권장합니다.; 이외에, bovine and porcine등 species와 반응할수 있습니다
  • p-Cofilin 1 (E-5): sc-271921무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGκ BP-HRP는 p-Cofilin 1 항체 (E-5) WB 및 IHC(P) 애플리케이션용입니다. 이 시약은 현재 p-Cofilin 1 항체 (E-5)(아래 주문 정보 참조)와 함께 번들로 제공됩니다. 추가 m-IgGκ BP 접합체는 마우스 IgG 결합 단백질 전체 목록을 참조하세요.

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p-Cofilin 1 항체(E-5)는 마우스, 쥐 및 인간 유래의 Ser 3 인산화 코필린 1을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgM κ 마우스 모노클로날 p-Cofilin 1 항체(p-Cofilin 1 항체 또는 코필린 항체로도 지정)이며 비접합 항-p-Cofilin 1 항체 형태로도 제공됩니다. LIM키나아제 1(LIMK-1)은 LIM 및 PDZ 도메인을 포함하는 세린/트레오닌 키나아제입니다. LIMK1은 시험관 내 및 생체 내에서 세린 3에서 코필린을 인산화합니다. 코필린은 액틴 분해 인자이며 액틴 세포 골격 재구성을 조절합니다. 세린 3에 대한 코필린의 인산화는 이러한 활동을 차단하는 것으로 알려져 있습니다. LIMK1 또는 다른 효소에 의한 ADF/코필린 단백질의 인산화는 결합된 ATP를 가수분해하고 인산염을 해리한 오래된 액틴 필라멘트를 절단하고 해중합하는 이러한 단백질의 능력을 억제하여 액틴 필라멘트를 안정화시키는 경향이 있습니다. 액틴 필라멘트의 빠른 회전율과 3차 메시워크 형성은 다양한 액틴 결합 단백질에 의해 조절됩니다. 따라서 코필린은 액틴 세포 골격 재배열을 유발하는 신호 캐스케이드의 말단 이펙터입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

p-Cofilin 1 항체 (E-5)

sc-271921
200 µg/ml
$316.00

p-Cofilin 1 (E-5): m-IgGκ BP-HRP 번들

sc-551996
200 µg Ab; 40 µg BP
$354.00

p-Cofilin 1 (E-5) 중화펩타이드

sc-271921 P
100 µg/0.5 ml
$68.00

Has this antibody been tested on frozen tissue sections for immunofluorescence, specifically mouse tissues? Are any antigen retrieval steps recommended?

Asked by: HB23
Thank you for your question. sc-271921 is recommended for mouse, rat, human , bovine , and porcine. We validate this antibody for WB, IP, IF, IHC(P) and ELISA. Please see attached protocol for antigen retrieval. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2023-03-16

Does this antibody (or any of the other anti-cofilin antibodies currently available) recognize Drosophila Cofilin by IF?

Asked by: JSR1
Thank you for your question. Sequence analysis shows very low to no homology between the epitope sequence of the antibodies and the target in Drosophila. If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing scbt@scbt.com or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Santa Cruz TS
Date published: 2021-07-07

What application is the blocking peptide sc-271921 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Anti-p-Cofilin per analisi in western blotL'Ab è stato testato utilizzando lisati cellulari MCF-7 e MDA-MD-231, ottenuti in RIPA buffer. 15 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A per 1h, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A per 1h. Il secondario 1:2000 in Blotto A per 1h. Il segnale ottenuto è pulito, con un'unica banda del perso molecolare atteso. La specificità del segnale è stata verificata utilizzando l'inibitore della chinasi ROCK, Y-27632.
Date published: 2024-07-23
Rated 5 out of 5 by from Great antibody for western blottingWe used 1:1000 on western blotting from NPCs, it give a nice and clear band.
Date published: 2018-07-19
Rated 5 out of 5 by from Great AbUsed a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. detected a very clean and strong band.
Date published: 2018-01-30
Rated 5 out of 5 by from Works very well on WBI used this antibody on brain tissue (protein load: 30 uL, Ab concentration: 1/1000), and it worked very well: one band and good intensity for quantification.
Date published: 2018-01-08
Rated 5 out of 5 by from The band is present and abundantThis antibody worked great with HEK293 whole cell lysis at 1:500 dilution and incubation with 5% BSA
Date published: 2017-11-09
Rated 5 out of 5 by from good antibodyI used it at 1:150 dilution with milk and worked very well.
Date published: 2017-04-14
Rated 5 out of 5 by from Sensitive and SpecificGreat specificity to phosphorylated Cofilin 1 as shown by phosphatase treatment.
Date published: 2017-01-16
Rated 5 out of 5 by from Very potent and highly specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and determined to be an excellent antibody for Western blotting. Strong detection of cofilin 1 was observed, but no cross-reactivities detected by Western blotting in HeLa cells exposed to anisomycin for 2 hours. No detection of the cofilin 1 target was observed in overnight-serum starved human A431 cells that were treated for 5 minutes with epidermal growth factor. However, there was no significant cross-reactivities on these A431 cell immunoblots with any off-target proteins.
Date published: 2016-12-08
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p-Cofilin 1 항체 (E-5) is rated 5.0 out of 5 by 11.
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