Otospiralin Antibody (A-12): sc-393239
- Otospiralin Antibody (A-12) is a mouse monoclonal IgG1 κ Otospiralin antibody provided at 200 µg/ml
- raised against amino acids 1-89 representing full length Otospiralin of mouse origin
- Otospiralin Antibody (A-12) is recommended for detection of Otospiralin of mouse, rat, human origin by WB, IP, IF and ELISA
- Anti-Otospiralin Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Otospiralin Antibody (A-12) for WB applications. These reagents are now offered in bundles with Otospiralin Antibody (A-12) (see ordering information below).
QUICK LINKS
SEE ALSO...
Otospiralin Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Otospiralin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Otospiralin antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Otospiralin, consisting of 89 amino acids, is specifically expressed in the inner ear and is encoded by the OTOS gene. Otospiralin is synthesized by fibrocytes located in the spiral limbus and spiral ligament of the cochlea, which play a crucial role in maintaining inner ear homeostasis. Proper functioning of these fibrocytes is essential, as any impairment can lead to significant auditory dysfunction. Notably, degeneration of fibrocytes due to absence of Otospiralin has been linked to irreversible deafness in guinea pigs and moderate hearing loss in mice. Additionally, downregulation of Otospiralin can contribute to loss of function in hair cells of the inner ear, highlighting its importance in auditory health. Otospiralin is evolutionarily conserved across species, from fish to mammals, and shares homology with gag p30 core shell proteins and SARS-related type C retroviruses, with one isoform produced through alternative splicing.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Otospiralin Antibody (A-12) References:
- Downregulation of otospiralin, a novel inner ear protein, causes hair cell degeneration and deafness. | Delprat, B., et al. 2002. J Neurosci. 22: 1718-25. PMID: 11880501
- Gene structure, chromosomal localization, and mutation screening of the human gene for the inner ear protein otospiralin. | Lavigne-Rebillard, M., et al. 2003. Neurogenetics. 4: 137-40. PMID: 12687421
- Gene expression profile of the mouse organ of Corti at the onset of hearing. | Pompeia, C., et al. 2004. Genomics. 83: 1000-11. PMID: 15177554
- Down-regulation of otospiralin mRNA in response to acoustic stress in guinea pig. | Caravelli, A., et al. 2004. Hear Res. 198: 36-40. PMID: 15567600
- Deafness and cochlear fibrocyte alterations in mice deficient for the inner ear protein otospiralin. | Delprat, B., et al. 2005. Mol Cell Biol. 25: 847-53. PMID: 15632083
- Characterization and development of an inner ear type I fibrocyte cell culture. | Gratton, MA., et al. 1996. Hear Res. 99: 71-8. PMID: 8970814
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Otospiralin Antibody (A-12) | sc-393239 | 200 µg/ml | $322.00 | |||
Otospiralin Antibody (A-12): m-IgG Fc BP-HRP Bundle | sc-530400 | 200 µg Ab; 10 µg BP | $361.00 | |||
Otospiralin Antibody (A-12): m-IgGκ BP-HRP Bundle | sc-523866 | 200 µg Ab, 40 µg BP | $361.00 | |||
Otospiralin Antibody (A-12): m-IgG1 BP-HRP Bundle | sc-544034 | 200 µg Ab; 20 µg BP | $361.00 | |||
Otospiralin Antibody (A-12) AC | sc-393239 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Otospiralin Antibody (A-12) HRP | sc-393239 HRP | 200 µg/ml | $322.00 | |||
Otospiralin Antibody (A-12) FITC | sc-393239 FITC | 200 µg/ml | $336.00 | |||
Otospiralin Antibody (A-12) PE | sc-393239 PE | 200 µg/ml | $349.00 | |||
Otospiralin Antibody (A-12) Alexa Fluor® 488 | sc-393239 AF488 | 200 µg/ml | $364.00 | |||
Otospiralin Antibody (A-12) Alexa Fluor® 546 | sc-393239 AF546 | 200 µg/ml | $364.00 | |||
Otospiralin Antibody (A-12) Alexa Fluor® 594 | sc-393239 AF594 | 200 µg/ml | $364.00 | |||
Otospiralin Antibody (A-12) Alexa Fluor® 647 | sc-393239 AF647 | 200 µg/ml | $364.00 | |||
Otospiralin Antibody (A-12) Alexa Fluor® 680 | sc-393239 AF680 | 200 µg/ml | $364.00 | |||
Otospiralin Antibody (A-12) Alexa Fluor® 790 | sc-393239 AF790 | 200 µg/ml | $364.00 |