NUBP2 Antibody (C-12): sc-376784

NUBP2 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects NUBP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NUBP2 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NUBP2, also known as nucleotide binding protein 2 or cytosolic Fe/S cluster assembly factor NUBP2, is a crucial 271 amino acid protein that plays a significant role in the assembly of iron-sulfur (Fe/S) clusters, which are vital for the function of numerous enzymes involved in critical cellular processes. NUBP2 is predominantly localized in the nucleus and cytoplasm, with notable presence at centrosomes during mitosis, and exhibits highest expression levels in skeletal muscle, fetal liver, lung, brain, and kidney tissues. NUBP2′s importance lies in essential function in extramitochondrial Fe/S protein maturation, where NUBP2 facilitates the transfer of a labile 4Fe-4S cluster to various apoproteins, thereby influencing cellular metabolism and energy production. NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins and is encoded by a gene located on human chromosome 16p13.3 and mouse chromosome 17 A3.3, highlighting evolutionary conservation and significance across species.

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NUBP2 Antibody (C-12) References:

1. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes.  |  Nakashima, H., et al. 1999. Genomics. 60: 152-60. PMID: 10486206
2. A novel eukaryotic factor for cytosolic Fe-S cluster assembly.  |  Roy, A., et al. 2003. EMBO J. 22: 4826-35. PMID: 12970194
3. Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis.  |  Stehling, O., et al. 2008. Mol Cell Biol. 28: 5517-28. PMID: 18573874
4. Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.  |  Okuno, T., et al. 2010. Mol Biol Rep. 37: 1165-8. PMID: 19263241
5. PTEN identified as important risk factor of chronic obstructive pulmonary disease.  |  Hosgood, HD., et al. 2009. Respir Med. 103: 1866-70. PMID: 19625176
6. A genome-wide association study identifies novel loci associated with circulating IGF-I and IGFBP-3.  |  Kaplan, RC., et al. 2011. Hum Mol Genet. 20: 1241-51. PMID: 21216879
7. The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.  |  Kypri, E., et al. 2014. Cell Mol Life Sci. 71: 517-38. PMID: 23807208
8. Nubp2 is required for cranial neural crest survival in the mouse.  |  DiStasio, A., et al. 2020. Dev Biol. 458: 189-199. PMID: 31733190
9. The expression of cuproptosis-related genes in hepatocellular carcinoma and their relationships with prognosis.  |  Zhao, X., et al. 2022. Front Oncol. 12: 992468. PMID: 36313717
10. Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker.  |  Abdulla, R., et al. 2023. Front Oncol. 13: 1167691. PMID: 37810966
11. Abnormal upregulation of NUBP2 contributes to cancer progression in colorectal cancer.  |  Lan, D., et al. 2025. Mol Cell Biochem. 480: 399-410. PMID: 38492158
12. NUBP2 deficiency disrupts the centrosome-check point in the brain and causes primary microcephaly.  |  Rushforth, R., et al. 2025. medRxiv.. PMID: 39867373

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NUBP2 Antibody (C-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-NUBP2 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NUBP2 Antibody (C-12) Specifications

Alternate Product Name: NUBP2

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 131-165

Epitope Target: NUBP2

Epitope Species: human

Product Reactivity/Detection: NUBP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for NUBP2 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin