NP60 Double Nickase Plasmid (h): sc-406940-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NP60 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NP60 Double Nickase Plasmid (h) and NP60 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLYR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NP60 Antibody (D-10): sc-390601
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NP60 Double Nickase Plasmid (h) | sc-406940-NIC | 20 µg | $410.00 | |||
NP60 Double Nickase Plasmid (h2) | sc-406940-NIC-2 | 20 µg | $410.00 |
Human GLYR1 encodes NP60, a nuclear protein that functions as a chromatin-associated regulator of transcription and genome maintenance. NP60 contains a glyoxylate reductase/hydroxypyruvate reductase-like domain and is reported to interact with chromatin and transcriptional complexes, linking metabolic-state sensing to epigenetic control. Through these activities, GLYR1 influences processes such as transcriptional regulation, DNA damage responses, and maintenance of nuclear architecture. Dysregulated GLYR1/NP60 activity has been associated in the literature with altered gene expression programs relevant to cancer biology and other disorders characterized by chromatin and genome instability.
NP60 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLYR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLYR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLYR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLYR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.