Date published: 2025-9-9

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eNOS 항체 (A-9): sc-376751

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데이터 시트
  • eNOS 항체 A-9 는 마우스 monoclonal IgG2a κ eNOS 항체, 117간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 NOS3의 내부에 위치한 1173-1202의 아미노산에 해당됩니다.
  • eNOS 항체 (A-9)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 NOS3 를 감지하는 데에 추천한다.
  • 항-eNOS 항체(A-9)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • eNOS (A-9): sc-376751무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGκ BP-HRP 는 eNOS 항체 (A-9) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다. 이 시약은eNOS 항체인 (A-9)와 세트로 제공드립니다。 (아래의 주문 정보를 참고해 주시길 바랍니다)。기타m-IgGκ BP 커플링제는,작은쥐 IgG 결합 단백질완정한 리스트를 참고 해 주시길 바랍니다.

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NOS3 항체(A-9)는 마우스, 쥐 및 인간 유래의 NOS3 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2a κ 마우스 모노클로날 NOS3 항체(eNOS 항체 또는 ecNOS 항체로도 지정됨)입니다. NOS3 항체(A-9)는 비접합 항-NOS3 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-NOS3 항체 모두로 제공됩니다. 산화질소(NO)는 광범위한 생물학적 활성을 가지고 있으며 계통 발생학적으로 다양한 종의 신호 전달 경로에 관여합니다. NO 합성을 담당하는 효소인 산화질소 합성효소(NOS)는 N-말단 산소분해효소 도메인과 C-말단 환원효소 도메인을 포함합니다. NOS의 활성에는 3개의 코기질(L-아르기닌, NADPH, O2)과 5개의 보조 인자 또는 보철 그룹(FAD, FMN, 칼모둘린, 테트라하이드로비옵테린 및 헴)뿐만 아니라 동이합체화가 필요합니다. 세 가지 다른 유전자의 산물을 나타내는 몇 가지 다른 NOS 이소형이 설명되었으며, 이는 세 가지 다른 유전자의 산물을 나타내는 것으로 밝혀졌습니다. 여기에는 뉴런에서 처음 활성이 확인된 NOS1(ncNOS라고도 함)과 내피 세포에서 처음 확인된 NOS3(ecNOS라고도 함)를 포함한 두 가지 구성적인 Ca2+/CaM 의존성 형태의 NOS가 포함됩니다. 유도 가능한 형태의 NOS인 NOS2(iNOS라고도 함)는 Ca2+와 무관하며 광범위한 세포 유형에서 발현됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

eNOS 항체 (A-9) 참고문헌:

  1. NOS3 조절: 신장 세뇨관 상피세포는 단순한 큰 내피세포가 아닙니다.  |  Pollock, JS. and Carmines, PK. 2006. Hypertension. 47: 19-21. PMID: 16344377
  2. Nos3-/- iPSCs 모델은 자궁 내 심장 병인의 일치하는 특징을 나타냅니다.  |  Campbell, KA., et al. 2015. J Mol Cell Cardiol. 87: 228-36. PMID: 26344701
  3. NOS3 다형성과 대퇴골두 골괴사 사이의 연관성.  |  Zhao, X., et al. 2019. Artif Cells Nanomed Biotechnol. 47: 1423-1427. PMID: 31007072
  4. NOS3 유전자 인트론 4 a/b 다형성은 상염색체 우성 다낭성 신장 질환 환자의 ESRD와 관련이 있습니다.  |  Padhi, UN., et al. 2022. J Bras Nefrol. 44: 224-231. PMID: 35138322
  5. SIRT6은 산화질소 합성효소 3(NOS3)을 조절함으로써 내피 의존성 이완을 조절합니다.  |  Wang, J., et al. 2023. Biochem Pharmacol. 209: 115439. PMID: 36720357
  6. 산화 질소의 상피 자가 독성: 백일해의 호흡기 세포 병리에서의 역할.  |  Heiss, LN., et al. 1994. Proc Natl Acad Sci U S A. 91: 267-70. PMID: 7506415
  7. 대식세포에서 NO 합성효소 유도에서 전사인자 IRF-1의 필요성.  |  Kamijo, R., et al. 1994. Science. 263: 1612-5. PMID: 7510419
  8. 산화 질소 합성 효소: 역할, 피해 및 제어.  |  Nathan, C. and Xie, QW. 1994. Cell. 78: 915-8. PMID: 7522969
  9. 산화질소 합성 효소: 구조와 촉매에 관한 측면.  |  Marletta, MA. 1994. Cell. 78: 927-30. PMID: 7522970
  10. 인간 면역결핍 바이러스 1형(HIV-1)에 감염된 단핵구에서 산화질소 합성효소 활성의 조절: HIV 관련 신경 질환에 대한 시사점.  |  Bukrinsky, MI., et al. 1995. J Exp Med. 181: 735-45. PMID: 7530762
  11. 직장에서 NO.  |  Schmidt, HH. and Walter, U. 1994. Cell. 78: 919-25. PMID: 7923361
  12. 산화질소는 생체 내에서 종양 제거 작용을 하는 중요한 매개체입니다.  |  Farias-Eisner, R., et al. 1994. Proc Natl Acad Sci U S A. 91: 9407-11. PMID: 7937779

주문정보

제품명카탈로그 번호 단위가격수량관심품목

eNOS 항체 (A-9)

sc-376751
200 µg/ml
$316.00

eNOS (A-9): m-IgGκ BP-HRP 번들

sc-523081
200 µg Ab, 40 µg BP
$354.00

eNOS 항체 (A-9) AC

sc-376751 AC
500 µg/ml, 25% agarose
$416.00

eNOS 항체 (A-9) HRP

sc-376751 HRP
200 µg/ml
$316.00

eNOS 항체 (A-9) FITC

sc-376751 FITC
200 µg/ml
$330.00

eNOS 항체 (A-9) PE

sc-376751 PE
200 µg/ml
$343.00

eNOS 항체 (A-9) Alexa Fluor® 488

sc-376751 AF488
200 µg/ml
$357.00

eNOS 항체 (A-9) Alexa Fluor® 546

sc-376751 AF546
200 µg/ml
$357.00

eNOS 항체 (A-9) Alexa Fluor® 594

sc-376751 AF594
200 µg/ml
$357.00

eNOS 항체 (A-9) Alexa Fluor® 647

sc-376751 AF647
200 µg/ml
$357.00

eNOS 항체 (A-9) Alexa Fluor® 680

sc-376751 AF680
200 µg/ml
$357.00

eNOS 항체 (A-9) Alexa Fluor® 790

sc-376751 AF790
200 µg/ml
$357.00

eNOS (A-9) 중화펩타이드

sc-376751 P
100 µg/0.5 ml
$68.00

有没有免疫荧光的相关图片

Asked by: zhaiyujie
感谢您的提问。在eNOS 抗体 (A-9): sc-376751的产品页面上,有WB和IHC的实验图片,目前没有IF的实验图片。但是,该产品目前有多篇引用文献,您可以尝试从这些引用文献中,查找IF的实验图片。这是该产品引用文献的链接:https://www.scbt.com/zh/p/nos3-antibody-a-9#citations. Thank you for your question. On the product page of eNOS antibody (A-9): sc-376751, there are experimental pictures of WB and IHC, but currently there are no experimental pictures of IF. However, this product currently has multiple citations, and you can try to find experimental images of IF from these citations. Here is a link to the references cited for the product: https://www.scbt.com/zh/p/nos3-antibody-a-9#citations.
Answered by: Technical Service SH
Date published: 2024-10-21

Has this antibody been raised against the native or denatured structure of the protein? Is this information available somewhere for all anitbodies?

Asked by: Jaideep Sahni
Thank you for your question. sc-376751 was ran under denaturing conditions. If an antibody is ran under reducing conditions it will be listed on the product page .
Answered by: BlakeJ
Date published: 2023-03-21

Hello, I am going to try WB for e-NOS- dimers and monomers. I found the article where they used sc-654 NOS3 Abs. Then I found that it has been replaced on sc-376751.Does it work the same? May I have a sample? Any suggestions for using LT-PAGE procedure?

Asked by: NiKa
Thank you for your question. Both the discontinued antibody and its successor have been raised against C-terminus of the protein. However, since the former is a polyclonal antibody, they are not identical. NOS3 Antibody (A-9): sc-376751 is recommended for detection of NOS3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Please feel free to reach out to our technical service team to arrange for a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2020-08-10

Does this Ab detect Ser-1177 phosphorylated form of nos3?

Asked by: Talita
Thank you for your question. This antibody has not been epitope mapped, but it does have an epitope surrounding Ser 1177. As long as the exact epitope isn't in the location of the phosphorylation, it will be able to detect both non-phospholylated and phosphorylated forms.
Answered by: Tech Service
Date published: 2021-09-20

Does sc-376751 work for IF for mouse tissue? If it does, what dilution would you suggest?

Asked by: LiHsien1
Thank you for your question. Yes, this antibody, sc-376751, NOS3 (A-9), is mouse reactive, and is recommended for IF. We recommend a starting dilution of 1:50 for IHC and IF use.
Answered by: Tech Service
Date published: 2020-08-06

what is the difference between NOS3 Antibody (A-9): sc-376751 and NOS3 Antibody (C-6) sc-376542 in immunohistochemical staining of cardiac tissue in rats and what is the recommended positive control and how antigen retrieval is performed?

Asked by: omaima ibrahim
Thank you for your question. These are very similar monoclonal antibodies for NOS3 that recognize distinct epitopes. We do not have any data specifically in rat cardiac tissue. However, the sc-376751: NOS3 (A-9) antibody has been published in rat cardiac samples several times for Western blotting, IF and IHC. For example, please see PMID: 29333212 and 30120384. We have used FFPE human pancreas and spleen as positive controls for IHC(P). The recommended antigen retrieval method was sodium citrate buffer (pH 6) with heat treatment. A link to our complete recommended protocol with details is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2019-08-30

Can you recommend a positive control to use with sc-376751: NOS3 (A-9) for Western blot?

Asked by: jenniferc
We recommend A-673: sc-2414, MCF7:sc-2206 or HUV-EC-C: sc-364180 whole cell lysates as we have great Western blot data showing specific detection of myogenin in these cell lines.
Answered by: Technical Support 10
Date published: 2020-08-06
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Rated 5 out of 5 by from Upregulation of IL-1 Receptor AntagonistUpregulation of IL-1 Receptor Antagonist by Aspirin in Glial Cells via Peroxisome Proliferator-Activated Receptor-Alpha
Date published: 2024-08-01
Rated 4 out of 5 by from Antibody for WBI tried this antibody for WB. It worked on brain tissue at 1:500
Date published: 2023-03-08
Rated 4 out of 5 by from Works wellWorks at a dilution of 1:500 on mouse lung lysates
Date published: 2021-02-18
Rated 4 out of 5 by from It works in westernI use 1:500 in WB of HUVECs,the band is not very clear,but it work in WB,I think it shoud use 1:100-200 in WB.
Date published: 2018-03-09
Rated 3 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Good antibody for WBStrong WB signal obtained using this antibody sample at 1:500 dilultion against HCAEC whole cell lysates. Good and quick service. Thanks.
Date published: 2017-05-19
Rated 5 out of 5 by from niceWe used this antibody to detect NOS3 in brain tissue of mouse by Western blot analysis and the results are amazing. If you use SDS-PAGE gel, we suggest that run the gel for 0.5 h at 40 V, then turn to 100V for 1-1.5h, you will get nice blot.
Date published: 2017-04-07
Rated 4 out of 5 by from great resultWe used this antibody(sc-376751) to detect NOS3 in mouse placenta by Western blot analysis and got a good result.
Date published: 2017-02-23
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eNOS 항체 (A-9) is rated 4.3 out of 5 by 17.
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