NHERF-1 Antibody (EBP-10): sc-51684
- NHERF-1 Antibody (EBP-10) is a mouse monoclonal IgG2b, cited in 2 publications, provided at 100 µg/ml
- raised against recombinant full length NHERF-1 of human origin
- recommended for detection of NHERF of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for NHERF-1 Antibody (EBP-10). This work is in progress.
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NHERF-1 Antibody (EBP-10) is a mouse monoclonal IgG2b antibody that detects NHERF-1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). NHERF-1, also known as EBP50, plays a crucial role in regulating the Na+/H+ exchange protein NHE3, which is essential for transepithelial sodium absorption primarily in the intestinal and renal brush border membranes. NHERF-1′s ability to interact with NHE3 through two PDZ domains allows NHERF-1 to act as a scaffold for a multiprotein complex that includes Ezrin, facilitating cAMP-mediated phosphorylation and subsequent inhibition of NHE3 activity. This regulation is vital for maintaining sodium homeostasis and fluid balance in the body. Additionally, NHERF-1′s amino-terminal PDZ domain is important for dimerization in vivo, which further influences regulatory functions. The phosphorylation of NHERF-1 at Ser 289 by G protein-coupled receptor kinase 6A highlights the intricate signaling pathways that modulate activity, while NHERF-1′s interaction with NHERF-2, also known as E3KARP, underscores the complexity of sodium transport regulation across different tissues. With specific reactivity and versatile applications, anti-NHERF-1 antibody (EBP-10) is an invaluable tool for researchers studying sodium transport mechanisms and related physiological processes.
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NHERF-1 Antibody (EBP-10) References:
- Oligomerization of NHERF-1 and NHERF-2 PDZ domains: differential regulation by association with receptor carboxyl-termini and by phosphorylation. | Lau, AG. and Hall, RA. 2001. Biochemistry. 40: 8572-80. PMID: 11456497
- Adenoviral expression of NHERF-1 in NHERF-1 null mouse renal proximal tubule cells restores Npt2a regulation by low phosphate media and parathyroid hormone. | Cunningham, R., et al. 2006. Am J Physiol Renal Physiol. 291: F896-901. PMID: 16705152
- Roles of NHERF1/EBP50 in cancer. | Georgescu, MM., et al. 2008. Curr Mol Med. 8: 459-68. PMID: 18781953
- The prognostic value of the Na⁺/ H⁺ exchanger regulatory factor 1 (NHERF1) protein in cancer. | Saponaro, C., et al. 2014. Cancer Biomark. 14: 177-84. PMID: 24878819
- Protein-DNA Interactions at the Opossum Npt2a Promoter are Dependent upon NHERF-1. | Clark, BJ., et al. 2016. Cell Physiol Biochem. 39: 1-12. PMID: 27322746
- NHERF1 Between Promises and Hopes: Overview on Cancer and Prospective Openings. | Centonze, M., et al. 2018. Transl Oncol. 11: 374-390. PMID: 29455084
- MINDIN Exerts Protumorigenic Actions on Primary Prostate Tumors via Downregulation of the Scaffold Protein NHERF-1. | Álvarez-Carrión, L., et al. 2021. Cancers (Basel). 13: PMID: 33498862
- Noncanonical Sequences Involving NHERF1 Interaction with NPT2A Govern Hormone-Regulated Phosphate Transport: Binding Outside the Box. | Mamonova, T. and Friedman, PA. 2021. Int J Mol Sci. 22: PMID: 33499384
- The Biological Relevance of NHERF1 Protein in Gynecological Tumors. | Sonnessa, M., et al. 2022. Front Oncol. 12: 836630. PMID: 35223518
- Astrocytic NHERF-1 Increases Seizure Susceptibility by Inhibiting Surface Expression of TREK-1. | Bae, Y., et al. 2024. Glia.. PMID: 39543986
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHERF-1 Antibody (EBP-10) | sc-51684 | 100 µg/ml | $322.00 |