NHERF-1 Antibody (6D364): sc-71698

NHERF-1 Antibody (6D364) is a mouse monoclonal IgG2b antibody that detects NHERF-1 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. NHERF-1, also known as EBP50, plays a crucial role in regulating the Na+/H+ exchange protein NHE3, which is essential for transepithelial sodium absorption primarily in the intestinal and renal brush border membranes. NHERF-1′s ability to interact with NHE3 through two PDZ domains allows NHERF-1 to act as a scaffold for a multiprotein complex that includes Ezrin, facilitating cAMP-mediated phosphorylation and subsequent inhibition of NHE3 activity. This regulation is vital for maintaining sodium homeostasis and fluid balance in the body. Additionally, NHERF-1′s amino-terminal PDZ domain is important for dimerization in vivo, which further influences regulatory functions. The phosphorylation of NHERF-1 at Ser 289 by G protein-coupled receptor kinase 6A highlights the intricate signaling pathways that modulate activity. Furthermore, NHERF-2, or E3KARP, is another protein that interacts with NHERF-1, underscoring the complexity of the regulatory networks involved in sodium transport. With specific reactivity and proven applications, anti-NHERF-1 antibody (6D364) is an invaluable tool for researchers studying sodium transport mechanisms and related physiological processes.

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NHERF-1 Antibody (6D364) References:

1. Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting.  |  Shenolikar, S., et al. 2002. Proc Natl Acad Sci U S A. 99: 11470-5. PMID: 12169661
2. Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells.  |  Lederer, ED., et al. 2003. J Am Soc Nephrol. 14: 1711-9. PMID: 12819230
3. kappa Opioid receptor interacts with Na(+)/H(+)-exchanger regulatory factor-1/Ezrin-radixin-moesin-binding phosphoprotein-50 (NHERF-1/EBP50) to stimulate Na(+)/H(+) exchange independent of G(i)/G(o) proteins.  |  Huang, P., et al. 2004. J Biol Chem. 279: 25002-9. PMID: 15070904
4. Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets.  |  Voltz, JW., et al. 2007. J Biol Chem. 282: 33879-33887. PMID: 17895247
5. Novel regulatory function for NHERF-1 in Npt2a transcription.  |  Khundmiri, SJ., et al. 2008. Am J Physiol Renal Physiol. 294: F840-9. PMID: 18216150
6. Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice.  |  Cunningham, R., et al. 2008. Am J Physiol Renal Physiol. 294: F1001-7. PMID: 18256311
7. NHERF1: molecular brake on the PI3K pathway in breast cancer.  |  Georgescu, MM. 2008. Breast Cancer Res. 10: 106. PMID: 18430260
8. Sodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney.  |  Weinman, EJ., et al. 2010. J Biol Chem. 285: 13454-60. PMID: 20200151
9. NHERF1 regulates actin cytoskeleton organization through modulation of α-actinin-4 stability.  |  Sun, L., et al. 2016. FASEB J. 30: 578-89. PMID: 26432781
10. Prognostic and therapeutic implications of NHERF1 expression and regulation in colorectal cancer.  |  Leiphrakpam, PD., et al. 2020. J Surg Oncol. 121: 547-560. PMID: 31867736

The preferred NHERF-1 antibody is NHERF-1 Antibody (A-7): sc-271552.