Date published: 2025-9-8

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NGFR p75 항체 (B-1): sc-271708

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데이터 시트
  • NGFR p75 항체 B-1 는 마우스 monoclonal IgG1 κ NGFR p75 항체, 40간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 NGFR p75의 내부에 위치한 393-427의 아미노산에 해당됩니다.
  • NGFR p75 항체 (B-1)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 NGFR p75 를 감지하는 데에 추천한다.
  • 항-NGFR p75 항체(B-1)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • NGFR p75 (B-1): sc-271708무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP는 NGFR p75 항체(B-1) WB 애플리케이션용입니다. 이 시약은 이제 NGFR p75 항체(B-1)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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NGFR p75 항체(B-1)는 마우스, 쥐 및 인간 유래의 NGFR p75 단백질을 WB, IP, IF 및 ELISA로 검출하는 IgG1 κ 마우스 단일 클론 NGFR p75 항체(NGFR 항체, p75NTR 항체, TNFRSF16 항체 또는 신경 성장 인자(NGF) 수용체 항체로도 지정됨)입니다. NGFR p75 항체(B-1)는 비접합 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항체로도 제공됩니다. Trk 종양 유전자는 막에 걸쳐 있는 단백질 티로신 키나아제인 gp140Trk를 암호화하며, 생체 내에서 발현이 신경 볏 기원의 감각 척수 및 뇌신경절의 뉴런으로 제한됩니다. 신경 성장 인자(NGF)는 신경 세포주와 배아 등쪽 뿌리 신경절에서 Trk A의 티로신 인산화를 자극합니다. NGF에 의한 Trk의 티로신 인산화는 빠르고 특이적이며 피코몰 양의 인자에서 발생하는데, 이는 반응이 생리학적 양의 NGF에 의해 매개된다는 것을 나타내며 Trk A가 NGF의 주요 신호 전달 메커니즘에 참여한다는 것을 시사합니다. Trk A 수용체 복합체의 추가 구성 요소인 NGFR p75는 낮은 친화력으로 신경 영양 인자에 결합하지만 효율적인 신호 전달에 필요합니다. NGFR p75는 Trk A 활성화를 촉진하고 리간드 복합체에 다운스트림 이펙터 분자를 모집할 수 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

NGFR p75 항체 (B-1)

sc-271708
200 µg/ml
$316.00

NGFR p75 (B-1): m-IgG Fc BP-HRP 번들

sc-529246
200 µg Ab; 10 µg BP
$354.00

NGFR p75 (B-1): m-IgGκ BP-HRP 번들

sc-522033
200 µg Ab, 40 µg BP
$354.00

NGFR p75 (B-1): m-IgG1 BP-HRP 번들

sc-543412
200 µg Ab; 20 µg BP
$354.00

NGFR p75 항체 (B-1) AC

sc-271708 AC
500 µg/ml, 25% agarose
$416.00

NGFR p75 항체 (B-1) HRP

sc-271708 HRP
200 µg/ml
$316.00

NGFR p75 항체 (B-1) FITC

sc-271708 FITC
200 µg/ml
$330.00

NGFR p75 항체 (B-1) PE

sc-271708 PE
200 µg/ml
$343.00

NGFR p75 항체 (B-1) Alexa Fluor® 488

sc-271708 AF488
200 µg/ml
$357.00

NGFR p75 항체 (B-1) Alexa Fluor® 546

sc-271708 AF546
200 µg/ml
$357.00

NGFR p75 항체 (B-1) Alexa Fluor® 594

sc-271708 AF594
200 µg/ml
$357.00

NGFR p75 항체 (B-1) Alexa Fluor® 647

sc-271708 AF647
200 µg/ml
$357.00

NGFR p75 항체 (B-1) Alexa Fluor® 680

sc-271708 AF680
200 µg/ml
$357.00

NGFR p75 항체 (B-1) Alexa Fluor® 790

sc-271708 AF790
200 µg/ml
$357.00

NGFR p75 (B-1) 중화펩타이드

sc-271708 P
100 µg/0.5 ml
$68.00

Hi, what is the best gel percentage for p75 to avoid having many bands? Also, which blocking solution do you use? BSA or Milk? And in which solution do you prepare the antibody?

Asked by: ka92
Thank you for your question. As the molecular weight of p75 is 75 kDa it is recommended to use a gel percentage of 10%. For blocking and antibody dilution we use UltraCruz® Blocking Reagent (sc-516214)
Answered by: Tech Support Europe
Date published: 2021-07-23

Can this antibody also be used for FACS?

Asked by: Libka
Thank you for your question. Anti-NGFR p75 Antibody (B-1) is available with fluorescent conjugates, which are suited for FACS.
Answered by: Tech Support Europe
Date published: 2021-07-14

Hi! I wonder which is the best method for WB analysis. In particular which blocking solution do you use?

Asked by: vale89
Thank you for your question. We recommend using a blocking buffer of 5% milk TBST for Western Blot. Our complete Western Blot protocol can be found on the website here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Service
Date published: 2017-07-13

What is the recommended fixation method for IF with NGFR p75 (B-1): sc-271708 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-02-03
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Rated 5 out of 5 by from Great AntibodyI used it in WB at 1:300 ratio and I obtained a strong and specific band.
Date published: 2022-06-06
Rated 5 out of 5 by from greatIt works great, the response is perfect, I get the results I want
Date published: 2022-02-03
Rated 5 out of 5 by from NGFR p75 antibody fo IHCI used this antibody for IHC on paraffin sectins. It works very well with a good positivecytoplasmic signal, anyway I recommend also the use of the corresponding blocking peptid, because it is possible to seea background signal.
Date published: 2020-10-02
Rated 4 out of 5 by from For me it's okThe antibody anti-NGFR p75 (B-1; sc-271708) was tested for Western blotting. The samples analyzed derive from human cancer cells known to be positive or negative for the p75 expression. As shown in the western blot, the antibody recognizes two bands: a specific band corresponding to 75 KDa (as expected) and an aspecific band at about 100 KDa (as expected). Western blot conditions: Blocking 5% Milk; Ab I 1:500 in tTBS Ab II 1:2000 anti-mouse in 3% Milk
Date published: 2019-05-31
Rated 4 out of 5 by from IF on mouse lung works fineImmunofluorescence staining on mouse lung works fine with this ab.
Date published: 2018-09-19
Rated 4 out of 5 by from Clear staining in western blotI used this antibody on primary brain culture and it works very well in western blot even at low concentrations (1:2000)
Date published: 2018-05-25
Rated 5 out of 5 by from Amazing Western blot and IHC resultsI tried several anti-p75NGFR antibodies (also produced by different companies). However, this antibody is became my first choice. We tested sc-271708 on rat brain and human tissues, and it gives a clear and strong signal at 75 kDa, which corresponds to the mature glycosylated form of the receptor. A band at about 60 kDa is also present, corresponding to the immature form of the receptor. Depending on the physiopathological conditions, it is also possible to observe p75 cleavage products.
Date published: 2017-07-14
Rated 5 out of 5 by from excellent antibodyI bought this antibody use for pig and rat peripheral nervous tissue cryosections immunofluorescence stain. We got perfect image results.
Date published: 2017-05-19
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NGFR p75 항체 (B-1) is rated 4.8 out of 5 by 16.
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