Date published: 2025-9-8

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NFκB p52/p100/NFKB2 항체 (C-5): sc-7386

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데이터 시트
  • NFκB p52/p100/NFKB2 항체 C-5 는 마우스 monoclonal IgG2a κ NFκB p52/p100/NFKB2 항체, 204간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노 산1-447 representing full length NFκB p52 of human origin
  • NFκB p52/p100/NFKB2 항체 (C-5)는 WB, IP, IF and FCM으로 mouse, rat and human유래의 NFκB p52 and p100 를 감지하는 데에 추천한다.
  • 항-NFκB p52/p100/NFKB2 항체(C-5)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • IF, IHC(P) 와 FCM에 사용가능한 Alexa Fluor® 405 결합제품이 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-7386 X, 200 µg/0.1 ml)이 있습니다.
  • NFκB p52/p100/NFKB2 (C-5): sc-7386무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP 는 NFκB p52/p100/NFKB2 항체 (C-5) WB 애플리케이션용입니다. 이 시약은 이제 NFκB p52/p100/NFKB2 항체 (C-5)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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NFκB p52 항체(C-5)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 유세포분석법(FCM)을 통해 쥐, 쥐, 인간에서 유래된 NFκB p52 단백질을 검출하는 마우스 단일클론 IgG2a 카파 경쇄 항체입니다. NFκB p52(C-5) 항체는 비접합 형태와 아가로스, 과산화효소, 피코에리트린, 플루오레세인 이소티오시아네이트, 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합체 형태로 제공됩니다. NF-κB p52는 면역 반응, 세포 생존, 염증 조절에 중요한 역할을 담당하기 때문에, NF-κB p52는 암과 자가면역질환을 포함한 다양한 질병을 이해하는 데 중요한 표적이 되고 있습니다. NF-κB p52는 전구체인 p105의 처리를 통해 생성되며, p65와 같은 다른 NF-κB 서브유닛과 이종이합체를 형성하여 표적 유전자의 전사를 활성화합니다. NFκB p52와 p65의 상호작용은 면역 및 염증 반응에 관여하는 유전자 발현에 필수적인 NFκB 복합체의 전사 활성을 강화합니다. NFκB p52의 기능과 상호작용을 이해하는 것은 병태생리학적 과정에서 NFκB p52의 역할을 밝히고 표적 치료를 개발하는 데 필수적입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

NFκB p52/p100/NFKB2 항체 (C-5) 참고문헌:

  1. 원발성 유방 상피 세포의 종양 괴사 인자 자극 성장에 NFkappaB1/p50이 필요하지 않음: NFkappaB2/p52 및 RelB에 대한 시사점.  |  Zhang, J., et al. 2007. Endocrinology. 148: 268-78. PMID: 17008396
  2. 후코이단은 B세포에서 C 엡실론 생식세포 전사 및 NFkappaB p52 전위를 방지하여 IgE 생성을 촉진합니다.  |  Oomizu, S., et al. 2006. Biochem Biophys Res Commun. 350: 501-7. PMID: 17027924
  3. p50-NF-카파 B와 상호 작용할 수 있는 새로운 Rel 계열 전사 활성화제인 RelB.  |  Ryseck, RP., et al. 1992. Mol Cell Biol. 12: 674-84. PMID: 1732739
  4. p65와의 시너지 효과로 HIV 전사를 자극하는 NF-카파 B 서브유닛의 복제.  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
  5. NF-kappa B의 p50 DNA 결합 서브유닛의 복제: rel 및 등쪽과 상동성.  |  Ghosh, S., et al. 1990. Cell. 62: 1019-29. PMID: 2203532
  6. NF-kappa B, KBF1, 등쪽 및 관련 사항.  |  Gilmore, TD. 1990. Cell. 62: 841-3. PMID: 2203533
  7. 관련 종양 유전자 및 세포 주기 모티프와 상동성을 갖는 카파 B DNA 결합 단백질을 코딩하는 미토겐 유도 유전자의 복제.  |  Bours, V., et al. 1990. Nature. 348: 76-80. PMID: 2234062
  8. 핵 인자 κB2 p52 단백질은 IκB 키나아제 엡실론 의존성 Sp1 단백질과 인터루킨 15의 유도를 통해 항바이러스 면역에 중요한 역할을 합니다.  |  Doyle, SL., et al. 2013. J Biol Chem. 288: 25066-25075. PMID: 23873932
  9. 활성 NF-카파B의 65-카파D 서브유닛은 I 카파B에 의한 NF-카파B의 억제를 위해 필요합니다.  |  Baeuerle, PA. and Baltimore, D. 1989. Genes Dev. 3: 1689-98. PMID: 2691328
  10. 여러 핵 인자가 면역글로불린 인핸서 서열과 상호 작용합니다.  |  Sen, R. and Baltimore, D. 1986. Cell. 46: 705-16. PMID: 3091258
  11. 유전적 인간 RelB 결핍은 감염에 대한 선천적 및 적응성 면역을 손상시킵니다.  |  Le Voyer, T., et al. 2024. Proc Natl Acad Sci U S A. 121: e2321794121. PMID: 39231201
  12. 고도로 발현된 p100/p52(NFKB2)는 인간 유방암 세포의 세포질에서 다른 NF-kappa B 관련 단백질을 격리합니다.  |  Dejardin, E., et al. 1995. Oncogene. 11: 1835-41. PMID: 7478612

주문정보

제품명카탈로그 번호 단위가격수량관심품목

NFκB p52/p100/NFKB2 항체 (C-5)

sc-7386
200 µg/ml
$316.00

NFκB p52/p100/NFKB2 (C-5): m-IgG Fc BP-HRP 번들

sc-525441
200 µg Ab; 10 µg BP
$354.00

NFκB p52/p100/NFKB2 (C-5): m-IgG2a BP-HRP 번들

sc-546256
200 µg Ab; 10 µg BP
$354.00

NFκB p52/p100/NFKB2 항체 (C-5) X

sc-7386 X
200 µg/0.1 ml
$316.00

NFκB p52/p100/NFKB2 항체 (C-5) AC

sc-7386 AC
500 µg/ml, 25% agarose
$416.00

NFκB p52/p100/NFKB2 항체 (C-5) HRP

sc-7386 HRP
200 µg/ml
$316.00

NFκB p52/p100/NFKB2 항체 (C-5) FITC

sc-7386 FITC
200 µg/ml
$330.00

NFκB p52/p100/NFKB2 항체 (C-5) PE

sc-7386 PE
200 µg/ml
$343.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 488

sc-7386 AF488
200 µg/ml
$357.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 546

sc-7386 AF546
200 µg/ml
$357.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 594

sc-7386 AF594
200 µg/ml
$357.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 647

sc-7386 AF647
200 µg/ml
$357.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 680

sc-7386 AF680
200 µg/ml
$357.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 790

sc-7386 AF790
200 µg/ml
$357.00

NFκB p52/p100/NFKB2 항체 (C-5) Alexa Fluor® 405

sc-7386 AF405
200 µg/ml
$357.00

Are you produce from the product with cat no. SC-7386 Which is NFkb a vial 10micro gram / 50 micro liter ?

Asked by: Ahmed 1412
Thank you for your question. To request a sample size please call or email technical service . (800)-457-3801 or scbt@scbt.com
Answered by: BlakeJ
Date published: 2023-06-21

I just ordered the NFkB2 antibody (C-5) sc-7386-AF647. Can you send a FCM intracellular staining protocol? Does it work with BD cyto/fix/perm and cyto/wash buffer?

Asked by: Anonymous
Thank you for your question. You can find our flow cytometry protocol under this link: https://www.scbt.com/resources/protocols/flow-cytometry We recommend using FCM Fixation Buffer (sc-3622) and FCM Wash buffer (sc-3624) for flow cytometry.
Answered by: Tech Support Europe
Date published: 2021-08-16

Are there citations of this antibody being used in flow cytometry?

Asked by: Vidhi
Thank you for your question. We are aware of one product citation using the PE conjugated version of this antibody (sc-7386 PE) for flow cytometry. Please refer to the following product citation on PubMed for more details, PMID: 19388934.
Answered by: Technical Support
Date published: 2021-01-27

Hi, I am just wondering the data sheet for NFκB p52 (C-5): sc-7386 has only a single band which is not p52 but looks like p100. why this western results is different from the one on its website? thanks

Asked by: NF worker
Thank you for your question. The Western blot data that is currently on the data sheet is not representative of the endogenous NFKB2 protein. This is Western blot data in mouse Nfkb2 transfected 293T whole cell lysate. This transfected lysate is overepressing the full length p100 protein.
Answered by: Technical Support
Date published: 2019-07-10

Is this antibody working with the secondary m-IgGκ BP-HRP (sc-516102)?

Asked by: Pipaón
Thank you for your question. This antibody should be OK to work with the mouse-IgGk BP-HRP. If you would like to try a sample of the monoclonal antibody or the mouse IgG binding protein, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-04-24

May I know what is the packaging volume does NFκB p52 Antibody (C-5) FITC (sc-7386 FITC; 200 µg/ml) come as? How many tests can be performed using flow cytometer? Thank you

Asked by: Jackie2017
Thank you for your question. We provide the FITC-conjugated primary antibody as 200 µg in 1 mL total volume. We recommend using approximately 1 µg of FITC conjugated primary antibody for each flow cytometry experiment (based on the parameters in our protocol which is provided below). We estimate that you can use the antibody for approximately 100-200 tests. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-01-23

The WB of the C-5 clone showed a strong band in P100, how can you use this clone for IF or IHC for NFkB2 activation, normally if P52 goes up , then P100 goes down, vice versa.

Asked by: mocky
Thank you for your question. The antibody will detect p100 and/or p52, depending on what is present in the sample at the time of antibody addition. If both are present, both will be detected and you would not be able to tell them apart. I would suggest also checking your sample for the presence of one or both forms before doing your IHC or IF experiment. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-10

How can this mouse monoclonal antibody be used for double staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-7386, is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 5 out of 5 by from Excellent NFκB p52/p100/NFKB2 antibodyIt works really well for WB, no background. Samples are sheep thymuses.
Date published: 2023-03-17
Rated 1 out of 5 by from Not detecting right sizeWe tested human cells with both monoclonal anti-p52 and polyclonal one, which discontinued. Monoclonal antibody detected a band at ~37kD, the polyclonal one detected two bands one is p100 and the small one is about 10kD.
Date published: 2019-07-10
Rated 5 out of 5 by from p52 works well in A375 cells.I used this antibody for IF staining in A375 cells. I works well.
Date published: 2018-03-02
Rated 5 out of 5 by from Western blotThis antibody work well in western blot (gastric cancer cell line: AGS, MKN45, dilute 1:1000)
Date published: 2017-11-14
Rated 5 out of 5 by from 非常好用这支NF-kB抗体真的非常好用,之前我购买过其他品牌的这个抗体,但是还没有申请的这个小样试用装好用,条带非常漂亮,特异性好。是一个不错的抗体选择。
Date published: 2017-10-15
Rated 5 out of 5 by from the antibody worked wellThis antibody(SC-7386) worked well on WB. Sample: rat liver Dilution ratio 1:1000
Date published: 2017-09-19
Rated 5 out of 5 by from popular usedNFκB p52 and NFκB p65 is very popular used in our research .This two antibody been used for WB detect Hela cell,always strong band by 1:1000 diluted the antibody.
Date published: 2017-06-25
Rated 5 out of 5 by from see clear bandI use Mouse brain tissue extracts , used at 1:1000,no background ,but clear band seen.
Date published: 2017-06-11
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NFκB p52/p100/NFKB2 항체 (C-5) is rated 4.8 out of 5 by 18.
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