Neurogenin 1 항체 (OR-7) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Neurogenin 1을 검출할것을 권장합니다.

Neurogenin 1 항체 (OR-7): sc-100332

Neurogenin 1 항체 (OR-7) is rated 3.0 out of 5 by 5.
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Datasheets

    • 안티-Neurogenin 1 항체 OR-7 는 마우스 monoclonal IgG2a (kappa light chain) Neurogenin 1 항체 이며 50 µg/0.5 ml으로 제공합니다.
    • human origin의 Neurogenin 1의 전체인 82-164 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Neurogenin 1을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • Neurogenin 1 (OR-7): sc-100332무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

I am using Neurogenin 1 (OR-7): sc-100332 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24

Has anyone try this antibody for immunoprecipitation of endogenous protein? Thanks!

Asked by: Raquel
Thank you for your question. We are not aware of anyone publishing with Neurogenin 1 Antibody (OR-7): sc-100332 for immunoprecipitation. We do warranty the antibody to work for this research application based on the performance of similar antibodies and the reactivity of this antibody in Western blot. Please contact our Technical Service Department for further support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Technical Support
Date published: 2017-01-19
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Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 1 out of 5 by from Did not work for immunohistochemistry I tried this antibody at a 1:50 and 1:500 dilution, with or without TSA-based signal amplification, on adult mouse brain sections (using blocking buffer with 0.3% Triton-X100, over-night incubation of primary at 4'C). It didn't work.
Date published: 2018-01-18
Rated 4 out of 5 by from This has worked well in IHC This has worked well in IHC.
Date published: 2015-10-27
Rated 4 out of 5 by from Nice band seen in WB using HeLa nuclear extract Nice band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-06-23
Rated 4 out of 5 by from Produced good immunofluorescence nuclear Produced good immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-02
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