Nek7 Antibody (B-5): sc-393539

Nek7 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nek7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nek7 antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nek7 protein plays a crucial role in regulating mitosis, particularly during the G2 to M phase transition, where Nek7 is essential for proper cell division. This regulation is vital for maintaining genomic stability and preventing the onset of diseases such as cancer, as aberrations in mitotic processes can lead to aneuploidy and tumorigenesis. Nek7 is predominantly localized in the cytoplasm and exhibits high expression levels in tissues such as lung, testis, muscle, spleen, heart, liver, leukocytes, and brain, while lower levels are found in the ovary, prostate, and kidney, with no detectable expression in the small intestine. The identification of Nek7′s specific interactions with other NIMA-related kinases further underscores Nek7′s importance in the mitotic process and cellular function, making Nek7 (B-5) monoclonal antibody a valuable tool for researchers studying cell cycle regulation and related pathologies.

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Nek7 Antibody (B-5) References:

1. Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators.  |  Tanaka, K. and Nigg, EA. 1999. J Biol Chem. 274: 13491-7. PMID: 10224116
2. NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3.  |  Chen, A., et al. 1999. Gene. 234: 127-37. PMID: 10393247
3. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3.  |  Kimura, M. and Okano, Y. 2001. Cytogenet Cell Genet. 94: 33-8. PMID: 11701951
4. A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells.  |  Letwin, K., et al. 1992. EMBO J. 11: 3521-31. PMID: 1382974
5. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.  |  He, Y., et al. 2016. Nature. 530: 354-7. PMID: 26814970
6. Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome.  |  Sharif, H., et al. 2019. Nature. 570: 338-343. PMID: 31189953
7. NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-κB signaling.  |  Chen, X., et al. 2019. Cell Death Dis. 10: 906. PMID: 31787755
8. Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase.  |  Osmani, SA., et al. 1988. Cell. 53: 237-44. PMID: 3359487
9. ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation.  |  Zheng, S., et al. 2023. Mol Cell. 83: 4570-4585.e7. PMID: 38092000
10. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans.  |  Schultz, SJ., et al. 1994. Cell Growth Differ. 5: 625-35. PMID: 7522034
11. The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes.  |  Rhee, K. and Wolgemuth, DJ. 1997. Development. 124: 2167-77. PMID: 9187143
12. Characterization of mammalian NIMA-related kinases.  |  Fry, AM. and Nigg, EA. 1997. Methods Enzymol. 283: 270-82. PMID: 9251026

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nek7 Antibody (B-5) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-Nek7 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nek7 Antibody (B-5) Specifications

Alternate Product Name: Nek7

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-26

Epitope Target: Nek7

Epitope Species: human

Product Reactivity/Detection: Nek7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for Nek7 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin