NBR1 Antibody (4BR): sc-130380

NBR1 Antibody (4BR) is a mouse monoclonal IgG1 kappa light chain antibody that detects NBR1 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-NBR1 antibody (4BR) is available as the non-conjugated format. NBR1, or neighbor of BRCA1 gene 1, is a 966 amino acid protein encoded by a gene adjacent to the well-known tumor suppressor BRCA1. NBR1 plays a crucial role in cellular processes, particularly in the regulation of autophagy and the degradation of misfolded proteins, which is vital for maintaining cellular homeostasis and preventing the accumulation of damaged proteins that can lead to various diseases, including cancer. NBR1 contains several structural motifs, such as a B-box/coiled coil domain, an OPR domain, and a ZZ-type zinc finger, which are characteristic of proteins involved in cell transformation. NBR1 interacts with key proteins like SQSTM1 (sequestosome 1), Titin, and MuRF2 (muscle-specific RING finger protein 2), indicating potential involvement in developmental pathways and tumor suppression. Two isoforms of NBR1, designated NBR1A and NBR1B, arise from alternative splicing, and their expression is downregulated in malignant mammary tissues, further underscoring NBR1′s significance in cancer biology.

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NBR1 Antibody (4BR) References:

1. Expression profiles and intergenic structure of head-to-head oriented Brca1 and Nbr1 genes.  |  Dimitrov, S., et al. 2001. Gene. 262: 89-98. PMID: 11179671
2. NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube.  |  Whitehouse, C., et al. 2002. Eur J Biochem. 269: 538-45. PMID: 11856312
3. Brca1 expression is regulated by a bidirectional promoter that is shared by the Nbr1 gene in mouse.  |  Whitehouse, C., et al. 2004. Gene. 326: 87-96. PMID: 14729266
4. Characterisation of a 161 kb deletion extending from the NBR1 to the BRCA1 genes in a French breast-ovarian cancer family.  |  Gad, S., et al. 2003. Hum Mutat. 21: 654. PMID: 14961556
5. Crystal structure of the PB1 domain of NBR1.  |  Müller, S., et al. 2006. FEBS Lett. 580: 341-4. PMID: 16376336
6. Sequential barriers and an obligatory metastable intermediate define the apparent two-state folding pathway of the ubiquitin-like PB1 domain of NBR1.  |  Chen, P., et al. 2008. J Mol Biol. 376: 1463-77. PMID: 18234223
7. Autophagy suppresses breast cancer metastasis by degrading NBR1.  |  Marsh, T. and Debnath, J. 2020. Autophagy. 16: 1164-1165. PMID: 32267786
8. Selective autophagy regulates heat stress memory in Arabidopsis by NBR1-mediated targeting of HSP90.1 and ROF1.  |  Thirumalaikumar, VP., et al. 2021. Autophagy. 17: 2184-2199. PMID: 32967551
9. The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation.  |  Zeng, Y., et al. 2021. PLoS Pathog. 17: e1009300. PMID: 33577621
10. LLPS of SQSTM1/p62 and NBR1 as outcomes of lysosomal stress response limits cancer cell metastasis.  |  Noguchi, T., et al. 2023. Proc Natl Acad Sci U S A. 120: e2311282120. PMID: 37847732
11. Enteric coronavirus nsp2 is a virulence determinant that recruits NBR1 for autophagic targeting of TBK1 to diminish the innate immune response.  |  Jiao, Y., et al. 2024. Autophagy. 20: 1762-1779. PMID: 38597182
12. Isolation and characterisation of the NBR2 gene which lies head to head with the human BRCA1 gene.  |  Xu, CF., et al. 1997. Hum Mol Genet. 6: 1057-62. PMID: 9215675

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NBR1 Antibody (4BR) has 53 citations in a variety of scientific publications.

Primary antibodies like Anti-NBR1 Antibody (4BR) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NBR1 Antibody (4BR) Specifications

Alternate Product Name: NBR1

Product Clone Name: 4BR

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-97

Epitope Target: NBR1

Epitope Species: human

Product Reactivity/Detection: NBR1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 53 citations in various scientific publications

Available Conjugates/Forms for NBR1 Antibody (4BR): N/A