Mucin 2/MUC2 Double Nickase Plasmid (h): sc-400221-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 2/MUC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mucin 2/MUC2 Double Nickase Plasmid (h) and Mucin 2/MUC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MUC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mucin 2/MUC2 Antibody (F-2): sc-515032
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 2/MUC2 Double Nickase Plasmid (h) | sc-400221-NIC | 20 µg | $410.00 | |||
Mucin 2/MUC2 Double Nickase Plasmid (h2) | sc-400221-NIC-2 | 20 µg | $410.00 |
MUC2 encodes mucin 2, a gel-forming secreted mucin that is a principal structural component of the intestinal mucus barrier and a key determinant of epithelial surface hydration and lubrication. Through extensive O-glycosylation and polymerization, MUC2 contributes to mucosal defense, regulates host–microbiota spatial separation, and supports epithelial homeostasis in the gastrointestinal tract. Its expression and processing intersect with goblet cell differentiation programs, ER/Golgi secretory pathway function, and mucin network assembly. Dysregulation of MUC2 abundance or mucin barrier properties is frequently investigated in inflammatory bowel disease, colitis-associated inflammation, and epithelial stress contexts relevant to colorectal tumor biology.
Mucin 2/MUC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MUC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MUC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MUC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MUC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.