Date published: 2025-9-7

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MEK-1/2 항체 (9G3): sc-81504

4.5(8)
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데이터 시트
  • MEK-1/2 항체 9G3 는 마우스 monoclonal IgG2a MEK-1/2 항체, 52간행물에 인용, 이며 50 µg/0.5 ml으로 제공합니다.
  • human의 MEK-1/2의 활성화 루프에 해당하는 포스포펩티드에 대해 제기됨
  • WB와 IP으로 mouse, rat, human와 canine origin의 the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status)을 검출할것을 권장합니다.
  • See MEK-1 (H-8): sc-6250 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • MEK-1/2 (9G3): sc-81504무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP 는 MEK-1/2 항체 (9G3) WB 애플리케이션용입니다. 이 시약은 이제 MEK-1/2 항체 (9G3)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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MEK-1/2 항체(9G3)는 마우스, 쥐, 인간 및 개 기원의 MEK-1/2 단백질을 WB 및 IP로 검출하는 IgG2a 마우스 단일 클론 MEK-1/2 항체(MAP2K1 항체, MAP2K2 항체 또는 미토겐 활성화 단백질 키나제 키나제 항체로도 지정됨)입니다. MEK-1/2 항체(9G3)는 비접합 항체로서 사용 가능합니다. MAP 키나아제의 상류에 위치하며 활성화를 담당하는 단백질 키나아제 계열이 확인되었습니다. 이 계열의 프로토타입 멤버인 MAP 키나아제 키나아제 또는 MEK-1은 ERK의 Thr-Glu-Tyr 모티프에 존재하는 MAP 키나아제 조절 트레오닌 및 티로신 잔기를 특이적으로 인산화합니다. 두 번째 MEK 계열인 MEK-2는 기질 특이성에서 MEK-1과 유사합니다. MEK-3(또는 MKK-3)은 p38 MAP 키나아제를 활성화하는 기능을 하며, MEK-4(SEK1 또는 MKK-4라고도 함)는 p38 및 JNK MAP 키나아제를 모두 활성화합니다. MEK-5는 ERK5를 특이적으로 인산화하는 것으로 보이는 반면, MEK-6는 p38 및 p38b를 인산화합니다. MEK-7(또는 MKK-7)은 JNK 신호 전달 경로를 인산화하여 활성화합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

MEK-1/2 항체 (9G3)

sc-81504
50 µg/0.5 ml
$316.00

MEK-1/2 (9G3): m-IgG Fc BP-HRP 번들

sc-539619
50 µg Ab; 10 µg BP
$354.00

MEK-1/2 (9G3): m-IgG2a BP-HRP 번들

sc-546742
50 µg Ab; 10 µg BP
$354.00

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-17
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Rated 4 out of 5 by from Not very clear blotDetected the bands but the film was quite unclear. Incubated the antibody in 3% v/w milk.
Date published: 2021-03-17
Rated 2 out of 5 by from please use this antibody with caution!I have previously recommended this antibody. I have thought this antibody can equally recognise the phosphor- and non-phosphor-form of MEK-1/2, according to the statement "recommended for detection of the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status)". However, recently I did a growth factor stimulation experiment. I suddenly realised that MEK-1/2 Antibody (9G3) appears to have a strong preference for the p-MEK-1/2 over non-p-MEK-1/2, but is not totally clean with non-p-MEK-1/2. Thus, it would be problematic to use this antibody, especially when the phosphorylation level is low. So, please use this antibody with caution!
Date published: 2017-10-26
Rated 5 out of 5 by from great for WBThis ab has very good specificity and sensitivity for WB with MEK-1/2 from human, mouse and rat. The epitope is in the activation loop, for sequential WB using a phosphor-MEK-1/2 and this Ab, whether the binding of one ab will interfere with the binding of the other is not unknown.
Date published: 2017-01-30
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of MEK-1/2 expression in serum starved HeLa, Hep G2, HEK293, SH-SY5Y, MDCK, PC-12, CMT 93, Neuro 2A whole cell lysates.
Date published: 2017-01-20
Rated 5 out of 5 by from Published data for IP and WB in MCF7 breastPublished data for IP and WB in MCF7 breast cancer cells. -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from Published data for WB in rat (VSMCs) vascularPublished data for WB in rat (VSMCs) vascular smooth muscle cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from Published data for IP in HEKPublished data for IP in HEK-293 cells. -SCBT Publication Review
Date published: 2015-03-13
Rated 5 out of 5 by from GoodGood, clear band visualized in WB in serum starved HeLa, Hep G2, HEK293, SH-SY5Y, MDCK, PC-12, CMT 93, Neuro 2A whole cell lysates. -SCBT QC
Date published: 2014-03-29
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MEK-1/2 항체 (9G3) is rated 4.5 out of 5 by 8.
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