Date published: 2025-9-9

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megalin/LRP2 항체 (H-10): sc-515772

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데이터 시트
  • megalin/LRP2 항체(H-10) 는 마우스 monoclonal IgG1κ, 26간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노 산 4581-4604 within a C-terminal cytoplasmic domain of megalin of rat origin 간의 피토프 매핑 에 특이적인다
  • 안티-megalin/LRP2 항체 (H-10)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 megalin 를 감지하는 데에 추천한다.
  • 항-megalin/LRP2 항체(H-10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • megalin/LRP2 (H-10): sc-515772무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 는 megalin/LRP2 항체 (H-10) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은megalin/LRP2 항체(H-10) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

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메갈린 항체(H-10)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 섹션 면역조직화학법(IHCP), 효소결합 면역흡착 분석법(ELISA) 등 다양한 응용법을 통해 쥐, 쥐, 인간 표본에서 메갈린을 검출하는 쥐 단일클론 IgG1 카파 경쇄 항체입니다. 메갈린(H-10) 항체는 비접합 형태와 아가로스, 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다양한 알렉사 플루오르® 접합체 등 여러 가지 접합체 형태로 제공됩니다. 메갈린(Megalin)은 저밀도 지단백질 수용체 유전자 계열에 속하는 단백질로서, LDLR, LRP, VLDLR, ApoER2와 같은 다른 수용체도 포함합니다. 메갈린은 주로 상피 세포의 정단막에 위치하며, 특히 근위 신장 세뇨관, 장, 뇌실막 세포에 집중되어 있습니다. 메갈린은 비타민 B12를 포함한 필수 분자의 재흡수에 중요한 역할을 합니다. 메갈린의 적절한 국소화는 알츠하이머병과 관련된 베타-아밀로이드 펩타이드의 제거와 관련된 아포지단백질 J(클러스터린이라고도 함)와 같은 다양한 리간드의 흡수를 매개하는 데 필수적입니다. 또한, 메갈린은 쥐의 하이만 신장염에서 항원 결정 인자 역할을 하여 신장 기능과 질병에서 메갈린의 중요성을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

megalin/LRP2 항체 (H-10)

sc-515772
200 µg/ml
$316.00

megalin/LRP2 (H-10): m-IgG Fc BP-HRP 번들

sc-528062
200 µg Ab; 10 µg BP
$354.00

megalin/LRP2 (H-10): m-IgG1 BP-HRP 번들

sc-533435
200 µg Ab; 20 µg BP
$354.00

megalin/LRP2 항체 (H-10) AC

sc-515772 AC
500 µg/ml, 25% agarose
$416.00

megalin/LRP2 항체 (H-10) HRP

sc-515772 HRP
200 µg/ml
$316.00

megalin/LRP2 항체 (H-10) FITC

sc-515772 FITC
200 µg/ml
$330.00

megalin/LRP2 항체 (H-10) PE

sc-515772 PE
200 µg/ml
$343.00

megalin/LRP2 항체 (H-10) Alexa Fluor® 488

sc-515772 AF488
200 µg/ml
$357.00

megalin/LRP2 항체 (H-10) Alexa Fluor® 546

sc-515772 AF546
200 µg/ml
$357.00

megalin/LRP2 항체 (H-10) Alexa Fluor® 594

sc-515772 AF594
200 µg/ml
$357.00

megalin/LRP2 항체 (H-10) Alexa Fluor® 647

sc-515772 AF647
200 µg/ml
$357.00

megalin/LRP2 항체 (H-10) Alexa Fluor® 680

sc-515772 AF680
200 µg/ml
$357.00

megalin/LRP2 항체 (H-10) Alexa Fluor® 790

sc-515772 AF790
200 µg/ml
$357.00

I've received a sample of this antibody and the expected molecular weight is around 600kDa, however post translational modification or other factors could result in a difference in observed weight. May I know what is the observed weight for this antibody?

Asked by: KXYC
Thank you for your question. We do have representative Western blot data on our website as a reference. In addition, you may want to check an additional reference, PMID: 11369806, which has observed detection ~280 kDa, non-reduced--MW varies depending on electrophoresis conditions used.
Answered by: Technical Support
Date published: 2019-04-13

Hi, what primary and secondary antibody concentration was used for the western blot pictured above?

Asked by: Mims
Thank you for your question. We use a 1:200 primary antibody dilution and a 1:1000 for the secondary reagent. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-10-12

Hi, is the antibody validation for IHC also performed on human kidney tissue?

Asked by: Muizenmeisje
If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 10
Date published: 2019-02-14

Can megalin (H-10): sc-515772 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, megalin (H-10): sc-515772 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great resultI have tested this antibody for flow cytometry on mouse kidney tissue (disaggregated using Liberase TM), and it worked perfectly. I also tested it for immunofluorescence on mouse kidney tissue, and it produced clean, high-quality images. Find the results attached.
Date published: 2025-06-20
Rated 5 out of 5 by from works great for mouse kidney IHC!using this antibody to do IHC of mouse kidney, it works great!
Date published: 2021-06-18
Rated 4 out of 5 by from megalin antibodygot this sample couple of weeks ago. It arrives quickly after our request. We are testing it and still trying to make it work in the RPE-1 cell line now.
Date published: 2017-10-11
Rated 4 out of 5 by from Good for immunofluorescence on mouse tissueGood signal by immunofluorescence on mouse kidney cryosections.
Date published: 2017-05-22
Rated 5 out of 5 by from Works great for Immunohistochemistrythis antibody works great for immunohistochemistry on paraffin fixed mouse tissues with sodium citrate as antigen retrieval. Here are the conditions, Blocking with MOM blocking reagent in 3% BSA, 1 hour at room temperature Overnight primary antibody incubation, 1:100 dilution; 1 hour goat anti mouse 1:500.
Date published: 2017-04-25
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megalin/LRP2 항체 (H-10) is rated 4.6 out of 5 by 5.
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