Mcm4 항체 (C-12) WB, IP, IF 와ELISA으로 y origin의 Mcm4을 검출할것을 권장합니다.

Mcm4 항체 (C-12): sc-166036

Mcm4 항체 (C-12) is rated 4.5 out of 5 by 2.
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데이터 시트

    • 안티-Mcm4 항체 C-12 는 마우스 monoclonal IgG2a (kappa light chain) Mcm4 항체 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 1-300 mapping at the N-terminus of Mcm4 of Saccharomyces cerevisiae origin
    • Mcm4 항체 (C-12)는 WB, IP, IF and ELISA으로 y유래의 Mcm4 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Mcm4 항체 (C-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Mcm4 (C-12): sc-166036무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Mcm4 Antibody (C-12) for WB applications. These reagents are now offered in bundles with Mcm4 Antibody (C-12) (see ordering information below).

We want to use Rad53 (sc-74429) and MCM4 (sc-166036). Do you give samples to test these antibodies? 

Asked by: Sathyan
Thank you for your question. We do offer a samples of sc-74427 and sc-166036. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-07

What blocking solution should I use with Mcm4 (C-12): sc-166036 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-12
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Rated 4 out of 5 by from The antibody worked! I got a positive result with the dilution factor 1:500 on a PVDF membrane. The band was relatively strong, so I might want to try to increase the dilution factor 1:1000 in next experiment.
Date published: 2017-03-19
Rated 5 out of 5 by from Positive results in Western blot analysis Positive results in Western blot analysis of yeast recombinant Mcm4 fusion protein. -SCBT QC
Date published: 2013-08-11
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