raised against amino acids 1-300 mapping at the N-terminus of Mcm4 of Saccharomyces cerevisiae origin
Mcm4 항체 (C-12)는 WB, IP, IF and ELISA으로 y유래의 Mcm4 를 감지하는 데에 추천한다 .
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Mcm4 항체 (C-12)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Mcm4 Antibody (C-12) for WB applications. These reagents are now offered in bundles with Mcm4 Antibody (C-12) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mcm4 Antibody (C-12) is a high quality monoclonal Mcm4 antibody (also designated Mcm4 antibody) suitable for the detection of the Mcm4 protein of Saccharomyces cerevisiae origin. Mcm4 Antibody (C-12) is available as both the non-conjugated anti-Mcm4 antibody form, as well as multiple conjugated forms of anti-Mcm4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Full-length Mcm4 protein sequence with immunogen highlighted:
We want to use Rad53 (sc-74429) and MCM4 (sc-166036). Do you give samples to test these antibodies?
Asked by: Sathyan
Thank you for your question. We do offer a samples of sc-74427 and sc-166036. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-07
What blocking solution should I use with Mcm4 (C-12): sc-166036 monoclonal antibody for Western blot?
Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
Rated 4 out of
The antibody worked!I got a positive result with the dilution factor 1:500 on a PVDF membrane. The band was relatively strong, so I might want to try to increase the dilution factor 1:1000 in next experiment.
Date published: 2017-03-19
Rated 5 out of
Positive results in Western blot analysisPositive results in Western blot analysis of yeast recombinant Mcm4 fusion protein. -SCBT QC