Matrilin-1 CRISPR/Cas9 KO Plasmid (h): sc-406830
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Matrilin-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Matrilin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Matrilin-1 CRISPR/Cas9 KO Plasmid (h) | sc-406830 | 20 µg | $397.00 |
Overview
MATN1 encodes matrilin-1, a cartilage-enriched extracellular matrix adaptor protein that helps organize the pericellular matrix by linking collagens and proteoglycans into supramolecular networks. Matrilin-1 contributes to chondrocyte differentiation, cartilage structural integrity, and extracellular matrix assembly during skeletogenesis and endochondral ossification. Through its modular domains and multimerization, it participates in matrix remodeling processes that influence tissue biomechanics and cell–matrix signaling. Altered MATN1 expression or matrix organization is studied in the context of cartilage degeneration and inherited or acquired skeletal and joint disorders where extracellular matrix homeostasis is disrupted.
Matrilin-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MATN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MATN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MATN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Matrilin-1 protein expression.
This CRISPR knockout system enables efficient generation of MATN1-deficient cell models for investigation of Matrilin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MATN1 exon(s) critical for Matrilin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MATN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Matrilin-1 CRISPR/Cas9 KO Plasmid (h) and Matrilin-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MATN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Matrilin-1 HDR Plasmid (h) and Matrilin-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MATN1 homology arms to support homology-directed repair at defined MATN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.