LHPP Double Nickase Plasmid (m): sc-429326-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LHPP Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LHPP Double Nickase Plasmid (m) and LHPP Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lhpp. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LHPP Antibody (B-2): sc-376648
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHPP Double Nickase Plasmid (m) | sc-429326-NIC | 20 µg | $410.00 | |||
LHPP Double Nickase Plasmid (m2) | sc-429326-NIC-2 | 20 µg | $410.00 |
Mouse Lhpp encodes LHPP, a histidine phosphatase that hydrolyzes phosphohistidine residues and contributes to the regulation of cellular phosphorylation balance. By modulating histidine phosphorylation, LHPP can influence signaling networks linked to metabolic homeostasis, cytoskeletal dynamics, and cell-cycle control. Altered LHPP activity has been associated in the literature with dysregulated growth signaling and metabolic reprogramming in disease-relevant contexts, supporting its study in models of tumor biology and tissue physiology. These features make Lhpp a useful target for dissecting phosphorylation-dependent pathways and their downstream transcriptional and phenotypic outputs.
LHPP Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lhpp locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lhpp. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lhpp function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lhpp-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.