Date published: 2025-9-9

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LAMP1 항체 (1D4B): sc-19992

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데이터 시트
  • LAMP1 항체 1D4B 는 랫드 monoclonal IgG2a, 280간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • PHA(phytohaemagglutinin)로 처리된 정상 림프구에 대해 항원화
  • 안티-LAMP1 항체 (1D4B)는 WB, IP, IF and FCM으로 mouse, rat and human유래의 LAMP-1 를 감지하는 데에 추천한다.
  • 항-LAMP1 항체(1D4B)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • IF, IHC(P) 와 FCM에 사용가능한 Alexa Fluor® 405 결합제품이 있습니다.
  • LAMP1 (1D4B): sc-19992무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 LAMP1 항체(1D4B)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

빠른 링크

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LAMP-1 항체(1D4B)는 웨스턴 블롯팅(WB), 면역침전(IP), 면역형광(IF), 유세포분석(FCM) 등의 응용을 통해 마우스, 랫트, 인간 샘플에서 LAMP-1을 검출하는 랫트 단일클론 IgG2a 항체입니다. 항-LAMP-1 항체(1D4B)는 비접합 형태와 아가로스, 호스래디시 퍼옥시다아제(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합 형태로 제공됩니다. CD107a로도 알려진 LAMP-1은 주로 리소좀 막에 위치한 당화형 I형 막 단백질로, LAMP-1은 리소좀의 완전성과 기능을 유지하는 데 중요한 역할을 합니다. LAMP-1의 적절한 국소화는 리소좀의 산도를 조절하고, 세포 항상성에 필수적인 자가소화를 막아 리소좀 막을 보호하는 데 도움이 됩니다. LAMP-1은 세포 부착과 면역 반응 조절을 포함한 다양한 세포 과정에 관여하는데, 특히 LAMP-1 발현이 증가하는 활성화된 혈소판에서 두드러지게 나타납니다. 이러한 LAMP-1의 증가는 이들 세포의 부착과 혈전 형성 특성을 촉진하는 데 기여하며, LAMP-1의 중요성을 정상적인 생리학뿐만 아니라 LAMP-1 발현이 현저하게 증가하는 리소좀 축적 장애와 같은 병리학적 상태에서도 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

LAMP1 항체 (1D4B) 참고문헌:

  1. LAMP-1 결핍 마우스의 정상적인 리소좀 형태와 기능.  |  Andrejewski, N., et al. 1999. J Biol Chem. 274: 12692-701. PMID: 10212251
  2. 아스파라긴 연결 올리고당은 램프-1과 램프-2를 세포 내 단백질 분해로부터 보호합니다.  |  Kundra, R. and Kornfeld, S. 1999. J Biol Chem. 274: 31039-46. PMID: 10521503
  3. LAMP-1/LAMP-2 이중 결핍 섬유아세포에서 콜레스테롤 교통은 방해받지만 단백질 분해 기능은 정상입니다.  |  Eskelinen, EL., et al. 2004. Mol Biol Cell. 15: 3132-45. PMID: 15121881
  4. 트랜스티레틴은 순환하는 리소좀 관련 막 단백질(LAMP-1)과 상호 작용합니다.  |  Chang, MH., et al. 2004. Biochem J. 382: 481-9. PMID: 15200388
  5. 수지상 세포-리소좀 관련 막 단백질(LAMP)과 LAMP-1-HIV-1 개그 키메라는 뚜렷한 세포 이동 경로를 가지며 다양한 에피토프 레퍼토리에 대한 T세포와 B세포의 반응을 촉진합니다.  |  Arruda, LB., et al. 2006. J Immunol. 177: 2265-75. PMID: 16887987
  6. 리소좀 관련 막 단백질인 LAMP-1 및 LAMP-2가 없는 경우 나이세리아 함유 파고좀의 성숙을 억제합니다.  |  Binker, MG., et al. 2007. Cell Microbiol. 9: 2153-66. PMID: 17506821
  7. 활성화 의존성 혈소판 표면 당단백질로서 LAMP-1의 확인 및 특성화.  |  Febbraio, M. and Silverstein, RL. 1990. J Biol Chem. 265: 18531-7. PMID: 2211717
  8. 리소좀 막 당단백질: LAMP-1 및 LAMP-2의 특성.  |  Chen, JW., et al. 1986. Biochem Soc Symp. 51: 97-112. PMID: 3101702
  9. 리소좀 막 당단백질 램프-1과 램프-2는 동원 가능한 세포 소기관에 존재하지만 인간 호중구의 호중구 과립에는 존재하지 않습니다.  |  Dahlgren, C., et al. 1995. Biochem J. 311 (Pt 2): 667-74. PMID: 7487911

주문정보

제품명카탈로그 번호 단위가격수량관심품목

LAMP1 항체 (1D4B)

sc-19992
200 µg/ml
$316.00

LAMP1 항체 (1D4B) AC

sc-19992 AC
500 µg/ml, 25% agarose
$416.00

LAMP1 항체 (1D4B) HRP

sc-19992 HRP
200 µg/ml
$316.00

LAMP1 항체 (1D4B) FITC

sc-19992 FITC
200 µg/ml
$330.00

LAMP1 항체 (1D4B) PE

sc-19992 PE
200 µg/ml
$343.00

LAMP1 항체 (1D4B) Alexa Fluor® 488

sc-19992 AF488
200 µg/ml
$357.00

LAMP1 항체 (1D4B) Alexa Fluor® 546

sc-19992 AF546
200 µg/ml
$357.00

LAMP1 항체 (1D4B) Alexa Fluor® 594

sc-19992 AF594
200 µg/ml
$357.00

LAMP1 항체 (1D4B) Alexa Fluor® 647

sc-19992 AF647
200 µg/ml
$357.00

LAMP1 항체 (1D4B) Alexa Fluor® 680

sc-19992 AF680
200 µg/ml
$357.00

LAMP1 항체 (1D4B) Alexa Fluor® 790

sc-19992 AF790
200 µg/ml
$357.00

LAMP1 항체 (1D4B) Alexa Fluor® 405

sc-19992 AF405
200 µg/ml
$357.00

O anticorpo em questão, está validado em mouse brain? (LAMP-1 ANTIBODY 1D4B)

Asked by: 1D4B
Thank you for your question. LAMP1 Antibody (1D4B): sc-19992 is recommended for detection of LAMP-1 of mouse, rat and human origin by WB, IP, IF and FCM. It can be used on mouse brain samples.
Answered by: Tech Support Europe
Date published: 2025-03-21

Hi! Is it possible to recognize the Lamp1 1D4B 488 with a secondary antibody or is the Alexa Fluor disturbing this process? Thank you.

Asked by: Karletz
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

is this antibody still available? Can I order it from thermofisher as a supplier? Thank you!

Asked by: Jing2
Thank you for your question. This LAMP-1 antibody is currently available. Please contact our Customer Care Department for further support.
Answered by: Technical Support
Date published: 2020-07-16

Hello I'm a researcher at City of Hope Medical Center. We are curious if the monoclonal antibody is targeting the glycan structure on LAMP-1 or the primary structure of the protein. Please let me know at your earliest convenience. Thx.

Asked by: Tong95
Thank you for your question. This antibody was raised against raised against NIH/3T3 mouse embryo fibroblast tissue culture cell membranes. The exact binding epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-02-18

Has sc-19992 been used for Western blots with the Licor system? I have not been able to detect Lamp1 in 293T/17 lysates with 1D4B, but have always been able to see Lamp1 with H4A3 on our Odyssey.

Asked by: Anonymous1
Thank you for your question. We have not specifically tested this LAMP-1 Antibody (1D4B): sc-19992 using the Licor Odyssey system for detection. But the antibody does work for Western blot, so we would not expect any difficulty using it in a system with infrared detection reagents. LAMP-1 Antibody (1D4B) is a rat monoclonal IgG2a, while the other clone, (H4A3) is a mouse monoclonal IgG1. Please check to see if the Licor-Odyssey secondary antibodies you are using are compatible with detection of both of these host species and isotypes. Please contact our Technical Service Department and we would be happy to assist you with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-12

I would like to get anti-rat Lamp-1 for IF. Are there papers using this antibody for IF? Could I get a sample?

Asked by: AAABBB
Thank you for your question. This particular LAMP-1 Antibody (1D4B): sc-19992 has been cited in 17 papers that we are aware of for use in IF. For example, PMID's: 15998735 and 23600861. For a complete list, you can search by product catalog number and research application (IF) on our website. See the link below for more information. https://www.scbt.com/scbt/misc/citationResults.jsp?_requestid=7296121 We do not currently offer a sample size of this antibody. This is an excellent well-established antibody and it is covered by our product warranty if you purchase it and have any trouble using it we can replace it or refund your purchase within one year of purchase. We do offer a 10 µg sample size of an alternative clone, LAMP-1 Antibody (H4A3): sc-20011. A link to the product page is below. This antibody is also suitable for IF on rat samples. Please contact our Technical Service Department if you would like to request a sample. https://www.scbt.com/scbt/product/lamp-1-antibody-h4a3?requestFrom=search
Answered by: Technical Support
Date published: 2017-02-23

What is the epitope recognized by LAMP-1 (1D4B)?

Asked by: cjMara
Thank you for your question. LAMP-1 Antibody (1D4B) is a rat monoclonal raised against NIH/3T3 mouse embryo fibroblast tissue culture cell membranes. The exact epitope recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 5 out of 5 by from Beautiful stainingBright signal was detected in immunocytostaining of cultured mouse neural cells.
Date published: 2024-03-29
Rated 4 out of 5 by from Good for IFImmunofluorescenceにおいてリソソームのマーカーとして使用しました。きれいにリソソームを染色することができました。
Date published: 2020-08-24
Rated 5 out of 5 by from works for WB and ICCWe got a sample and then purchased a full size. Antibody is looking fine both for Western blot and ICC
Date published: 2019-04-16
Rated 5 out of 5 by from Great antibodyUsed for western blotting. Dilution 1:1000, 1% skimmed milk in TBST, HT22 cell lysate (20ug)
Date published: 2019-03-25
Rated 5 out of 5 by from Good antibody!I use this for IF analyses in the Hinck lab, and I see great detection. I have also used the conjugated form (680), and it works great in combination with other SCBT conjugated antibodies.
Date published: 2018-05-09
Rated 5 out of 5 by from Very good Ab for WBI got free sample of this Ab from Santa Cruz and Tested it on mouse liver tissue and works very well. One single band as expected.
Date published: 2018-04-26
Rated 5 out of 5 by from My Review for LAMP-1 Antibody (1D4B)Excellent antibody for western blotting ( 1:500 in 5% BSA) and Immunofluorescence ( 1:50 on methanol fixed cells/ FFPE epidermis ) in primary mouse keratinocytes and mouse epidermal tissue
Date published: 2018-03-18
Rated 5 out of 5 by from great antibodyI used this antibody for mouse heart frozen tissue staining. It worked very beautiful.
Date published: 2018-01-22
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LAMP1 항체 (1D4B) is rated 4.5 out of 5 by 22.
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