Date published: 2025-9-7

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KRAS 항체 (F234): sc-30

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데이터 시트
  • KRAS 항체 F234 는 마우스 monoclonal IgG2a κ KRAS 항체, 459간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • K-Ras의 rat 기원의 재조합 아미노산 54-189에 대한 항체 생성
  • WB, IP와 IF으로 mouse, rat와 human origin의 c-K-Ras and v-K-Ras을 검출할것을 권장합니다.; c-H-Ras and c-N-Ras와 교차반응이 있을수 있습니다.
  • See pan Ras (C-4): sc-166691 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • KRAS (F234): sc-30무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGκ BP-HRP는 KRAS 항체 (F234) WB 애플리케이션용입니다. 이 시약은 현재 KRAS 항체 (F234)(아래 주문 정보 참조)와 함께 번들로 제공됩니다. 추가 m-IgGκ BP 접합체는 마우스 IgG 결합 단백질 전체 목록을 참조하세요.

빠른 링크

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K-Ras 항체 (F234)는 WB, IP 및 IF에 의해 마우스, 랫트 및 인간 출처의 K-Ras 단백질을 검출하는 IgG2a κ 마우스 단일 클론 K-Ras 항체 (KRAS 항체 또는 c-K-Ras 항체로도 지정됨)이다. K-Ras 항체 (F234)는 비결합 항-K-Ras 항체로 사용될 수 있다. 포유류 Ras (v-Ha-Ras, Harvey rat sarcoma viral oncogene homogene, HRAS1, K-Ras, N-Ras1 또는 c-bas/has) 유전자 계열은 각각 (c-H-Ras2 및 c-K-Ras1) 및 N-Ras 유전자의 비활성 유사 유전자인 Harvey 및 Kirsten Ras 유전자 (c-H-Ras1 및 c-K-Ras2)로 구성된다. 세 개의 Ras 종양 유전자인 H-Ras, K-Ras 및 N-Ras는 GTP/GDP 결합 및 GTPase 활성을 갖는 단백질을 인코딩한다. Ras 단백질은 GDP에 결합된 비활성 형태와 GTP에 결합된 활성 형태 사이에서 번갈아 가며 구아닌 뉴클레오티드 교환 인자 (GEF)에 의해 활성화되고 GTPase 활성 단백질 (GAP)에 의해 비활성화된다. Ras 명명법은 쥐에서 유래한 쥐 육종 바이러스 (HaMSV)의 하비 균주인 C형 포유류 레트로바이러스의 발암 서열을 포함하는 복제된 DNA 단편과 상동인 인간 DNA 서열의 특성화에서 비롯된다. 정상적인 조건에서 Ras 계열 구성체는 세포하막 구획화 기반 신호 시스템에서 세포 성장 및 분화 이벤트에 영향을 미친다. 발암형 Ras는 세포 증식과 세포 자멸을 모두 제어하는 프로세스를 탈규제할 수 있다. GTP 가수분해 결합 신호 전달 단백질의 Ras 슈퍼 계열은 Ras, Rho, Rab 및 ARF 계열로 세분화될 수 있다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

KRAS 항체 (F234) 참고문헌:

  1. Ras의 어두운 면: 세포사멸 조절.  |  Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
  2. 전립선암 진행에서의 Ras 신호.  |  Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
  3. 인간 RAS 수퍼패밀리 단백질 및 관련 GTPase.  |  Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
  4. 종양 진행 및 전이에서의 발암성 Ras.  |  Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
  5. Ras 원형질막 신호 전달 플랫폼.  |  Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
  6. 골지에서의 Ras 신호 전달.  |  Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
  7. 구획화된 Ras/MAPK 신호.  |  Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
  8. Ras, PI(3)K 및 mTOR 신호는 종양 세포 성장을 제어합니다.  |  Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
  9. 포유류 육종 레트로바이러스의 형질 전환 유전자와 관련된 인간 DNA의 세 가지 유전자.  |  Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598

주문정보

제품명카탈로그 번호 단위가격수량관심품목

KRAS 항체 (F234)

sc-30
100 µg/ml
$316.00

KRAS (F234): m-IgGκ BP-HRP 번들

sc-520388
100 µg Ab, 40 µg BP
$354.00

Is this antibody specific for KRAS 4A or KRAS 4B or does it recognise both isoforms?

Asked by: CristinaM
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2022-03-14

对于KRAS G12D突变的情况。这个抗体能反映出 G12D与非突变的差异吗

Asked by: HRZ_Richard
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 9
Date published: 2022-03-14

Can this SC-30 antibody be used for flow cytometry purposes? And if so, what protocol would you recommend using (in terms of obtaining the appropriate amount of antibody for flow sorting human cancer cells)? 

Asked by: MA08
Thank you for your question. Here is a link to the protocol we use for Flow Cytometry: https://www.scbt.com/scbt/resources/protocols/flow-cytometry If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-16

Can anti-kras antibodies be used during immunofluorescence assays of cell cultures without a permeabilization step? In other words, can k-ras be detected and recognized from outside the cell?

Asked by: cicak
Thank you for your question. K-Ras is generally a cytoplasmic membrane protein, which requires the antibody to be able to enter the cell. We recommend methanol fixation as this partially permeabilizes the cell allowing for good detection. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-05-08

IS THIS CAN BE USE FOR IHC AT HUMAN TISSUE SAMPLE (PARAFINATED)

Asked by: DIKSHA
Thank you for your question. We have not tested this mouse monoclonal, K-Ras (F234): sc-30, for IHC on formalin-fixed paraffin-embedded tissue sections. However, this particular clone has been cited in several publications for this research application so it may be suitable. As a reference, please refer to the following product citations on PubMed, PMID's: 12727811, 10884918, 18973197 and 19545448.
Answered by: Technical Support
Date published: 2017-01-10

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
No, since this antibody doesn't have any direct conjugates available, it would not be recommended for IHC in mouse tissue. See pan Ras (C-4): sc-166691 for K-Ras antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Tech Service 5
Date published: 2016-12-16

hi, what dilution of this antibody to be used for western blot?

Asked by: moni
Thank you for your question. We recommend using this mouse monoclonal, K-Ras Antibody (F234): sc-30, at a starting dilution of 1:200 (range of 1:100-1:1,000) for Western blotting. This information is also provided on our product datasheet. A link is posted below. https://datasheets.scbt.com/sc-30.pdf
Answered by: Technical Support
Date published: 2020-02-06
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Rated 5 out of 5 by from Great signalI have used this antibody many time in blots. Works all the time. I recommend.
Date published: 2024-05-30
Rated 5 out of 5 by from Great K-Ras antibody for Western Blot applicationClone F234 is a classic K-ras antibody raised against the rat protein, but works well with human K-Ras as well. Antibody showed a strong 21 KDa Western Blot band with transfected lysate sc-111225, that over-expressed human K-Ras (right lane) Detected with mouse IgG kappa binding protein (m-IgGκ BP): sc-516102 GenBank Sequence Reference: BC013572.2 -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from K-Ras 抗体 (F234) 做WB效果超级棒K-Ras 抗体 (F234)买来做WB的,和其他的品牌比起来效果太好了。超赞,一如既往的支持Santa Cruiz抗体,科研工作者的首选。
Date published: 2021-11-09
Rated 3 out of 5 by from good for overexpressed proteinI used it with milk overnight at 4oC but detected only the overexpressed protein from A549 lung adenocarcinoma cells
Date published: 2021-03-17
Rated 4 out of 5 by from Figura 2 - J, KWestern blot analysis of KRAS in three mutant KRAS lung adenocarcinoma cell lines (H1792.604, H2009, and H358).
Date published: 2019-02-22
Rated 4 out of 5 by from A very specific antibodyI requested the free sample and the result was good.
Date published: 2018-07-29
Rated 1 out of 5 by from Ne détecte pas la protéineSur HEK transfectées pour surexprimer K-Ras4B, aucun signal détecté dans les extraits totaux (WB réalisé en PBS). Sur cette même manip, l'IP réalisée avec cet anticorps n'a permis de détecter aucun signal de Ras.
Date published: 2018-03-18
Rated 3 out of 5 by from KRas WBWe bought this antibody to do Western blots for endogenous KRas in HEK and HeLa cells. Endogenous KRas levels in those cell lines are rather low so while we can detect it, there are several strong non-specific protein bands, which is slightly dispappointing. Otherwise, not a bad antibody.
Date published: 2018-03-08
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KRAS 항체 (F234) is rated 3.9 out of 5 by 31.
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