human origin의 IQGAP3에서 에 가까운 538-592 아미노산을 항원으로 사용하였습니다.
안티-IQGAP3 항체 (D-10)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 IQGAP3 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-IQGAP3 항체 (D-10)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IQGAP3 Antibody (D-10) for WB applications. These reagents are now offered in bundles with IQGAP3 Antibody (D-10) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
IQGAP3 Antibody (D-10) is a high quality monoclonal IQGAP3 antibody suitable for the detection of the IQGAP3 protein of mouse, rat and human origin. IQGAP3 Antibody (D-10) is available as both the non-conjugated anti-IQGAP3 antibody form, as well as multiple conjugated forms of anti-IQGAP3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. IQGAP3 (IQ motif containing GTPase activating protein 3) is a 1,631 amino acid protein that acts as an effector of Cdc42 and Rac 1, linking their activation to the cytoskeleton during neuronal morphogenesis. A novel member of the IQGAP family, IQGAP3 is highly expressed in brain where it localizes to axons of hippocampal neurons. IQGAP3 contains one Ras-GAP domain, a CH (calponin-homology) domain, four IQ domains and is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson′s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 1 out of
TerribleUsed 100 ug of tissue on a western blot and could barely detect anything. Probed for GAPDH on this same membrane and it was fine so I know it wasn't the blot.
Date published: 2018-02-21
Rated 1 out of
detect only IQ2overexpressed GFP-IQ1, 2, 3 in 293 cells. the Ab could detect only IQ2 weakly. there is weak non-specific band on 100kD.
1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-23
Rated 5 out of
Very good Western blot data of IQGAP3 expressionVery good Western blot data of IQGAP3 expression in Hep G2 whole cell lysate and human placenta tissue extract . -SCBT QC