Iodonitrotetrazolium chloride (CAS 146-68-9)

사용법 :

spectrophotometric assay for succinate dehydrogenase activity:

Materials:

spectrophotometer
cuvette with 1-cm light patch.

Reaction Mixture:
100 mmol/liter triethanolamine (HCl), pH8,3
o.5 mmol/liter EDTA,
2 mmol/liter Iodonitrotetrazolium chloride,
12 g/liter Cremophor EL,
20 mmol/liter succinate, pH7.4

Reaction:
mix 990 ul of reaction mixture with 10ul mitochondrial suspension
color develops at 30°C
record for 6 min. @500 nm

모습 :

Powder

건강 상태 :

Solid

용해도 :

Soluble in water (4 mg/ml), ethanol (8 mg/ml), ethylene glycol (30 mg/ml), DMSO (slightly), and methanol: water (1:1, 50 mg/ml, very faintly turbid, very deep yellow,hot).

저장 :

Store at room temperature

녹는점 :

240° C (dec.)(lit.)

IC50 :

CHO: IC₅₀ = 49 µM (cricetulus griseus)

WGK 독일 :

3

PubChem CID :

MDL 번호 :

MFCD00011961

EC 번호 :

205-676-2

Beilstein 기재 :

4093224

SMILES :

C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=C(C=C3)I)C4=CC=C(C=C4)[N+](=O)[O-].[Cl-]