insulin R beta 항체 (CT-3) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 insulin Rβ을 검출할것을 권장합니다.

insulin Rβ 항체 (CT-3): sc-57342

insulin Rβ 항체 (CT-3) is rated 4.3 out of 5 by 18.
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Datasheets

    • 안티-insulin R beta 항체 CT-3 는 마우스 monoclonal IgG1 (kappa light chain) insulin Rβ 항체 이며 200 µg/ml으로 제공합니다.
    • C-terminus of insulin receptor of human origin의하고 제작된다
    • 안티-insulin R beta 항체 (CT-3)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 insulin Rβ 를 감지하는 데에 추천한다.; insulin-like growth factor (IGF)receptors와 교차반응이 없습니다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-insulin R beta 항체 (CT-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • insulin Rβ (CT-3): sc-57342무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
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Is a blocking pepide available for the sc-57342 Insulin receptor antibody?

Asked by: PaulDA
Thank you for your question. There is no blocking peptide available for this specific antibody.
Answered by: Technical Support
Date published: 2019-09-11

Can you provide information about the hybridoma cell line this antibody was produced from?

Asked by: KMitok
Thank you for your question. The specific clone name for this monoclonal antibody is CT-3. If you require additional information, please contact our Technical Service Department for assistance.
Answered by: Technical Support
Date published: 2019-09-11

I have a clear band of approximately 45 KDa with this antibody. Do you know what it can be? Thank you so much!

Asked by: Jimena
Thank you for your question. We do not have any information regarding a 45 kDa sized band when using this antibody. Please contact our Technical Support Department for help with troubleshooting the antibody, or more detailed information about the expected molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

In the data sheet , it was written the antigen is C-terminal , does the C-terminal region contain Tryosine domain?

Asked by: TANG QIAN
Thank you for your question. This antibody was made against a recombinant protein fragment including the C-terminal 100 amino acid residues of human insulin receptor (accession#P06213). There are tyrosine domains within the C-terminus but since the antibody has not been epitope mapped, I do not know if the exact epitope includes a Tyrosine. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with insulin Rβ (CT-3): sc-57342 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-10
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Rated 4 out of 5 by from Application of insulin Rβ Antibody for IHC and WB The insulin Rβ Antibody (CT-3) SC-57342 is suitable for intracellular localization studies of the insulin receptor in 3T3-L1 adipocytes at different stages of differentiation by immunofluorescence and confocal microscopy . The antibody can be also used for Western blot studies of protein extracts from 3T3-L1 adipocytes, including quantitative analyses.
Date published: 2018-09-13
Rated 5 out of 5 by from Works great! Works at 1:50 dilution for immunofluorescence in hES cells
Date published: 2018-03-01
Rated 5 out of 5 by from Works great for IHC in frozen mouse liver sections I used this at a 1:250 dilution on frozen mouse liver sections and it worked great! Did not work well in paraffin embedded sections.
Date published: 2018-01-03
Rated 4 out of 5 by from nice band the antibody was tested in western blot on rat tissue samples and worked well
Date published: 2017-12-13
Rated 4 out of 5 by from positive result in WB worked well for detection of insulin receptor from cell lysate
Date published: 2017-09-06
Rated 3 out of 5 by from ISR b This antibody was used for Western blotting of MCF7 and T47D cell lysate。It did not work in T47D。It had some signal with a little background in MCF7.
Date published: 2017-06-15
Rated 4 out of 5 by from relative high sensitivity show some cross reaction with IGF-1Rb, but generally ok to detect IRb
Date published: 2017-05-17
Rated 1 out of 5 by from Confronto tra anticorpo poli e monoclonale A parità di proteine caricate (40ug) e condizioni di diluizione anticorpo (1:1000 in milk 2.5%) il nuovo anticorpo monoclonale (sc-57342) non funziona in modo soddisfacente rispetto al vecchio anticorpo policlonale (sc-711).
Date published: 2017-04-03
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