Date published: 2025-9-10

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Insulin B 항체 (C-12): sc-377071

4.8(12)
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데이터 시트
  • Insulin B 항체 C-12 는 마우스 monoclonal IgG1 κ Insulin B 항체, 23간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 Insulin B에서 N-terminus에 위치한 33-69의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • Insulin B 항체 (C-12)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Insulin B chain 를 감지하는 데에 추천한다.; 이외에, equine, canine, bovine and porcine등 species와 반응할수 있습니다
  • 항-Insulin B 항체(C-12)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • WB, IHC(P) 와 ELISA에 사용가능한 biotin결합제품 이 있습니다.
  • Insulin B (C-12): sc-377071무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 Insulin B 항체 (C-12) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Insulin B 항체(C-12) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

더보기

인슐린 B 항체(C-12)는 마우스, 쥐 및 인간 유래의 인슐린 B 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 단일 클론 인슐린 B 항체(인슐린 펩티드 항체, 인슐린 성장호르몬 항체로도 지정됨)입니다. 인슐린 B 항체(C-12)는 비접합 항-인슐린 B 항체 형태와 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-인슐린 B 항체 형태로 제공됩니다. 인슐린은 분비되는 펩티드 호르몬으로 포도당 흡수 증가 및 글리코겐 합성과 같은 대사 효과를 유도하여 혈당 농도를 낮추는 역할을 합니다. 인슐린은 먼저 전구체 분자인 프리프로인슐린으로 형성되며, 이후 프로인슐린으로 분해되어 최종적으로 성숙한 인슐린 호르몬이 됩니다. 성숙한 인슐린은 51개의 아미노산으로 구성되며, 두 개의 이황화 브릿지로 연결된 A 사슬과 B 사슬에 포함되어 있습니다. 인슐린은 단당류, 아미노산 및 지방산에 대한 세포 투과성을 증가시킵니다. 인슐린은 외인성 자극이 없을 때 췌장에서 기저 수준에서 분비되며 포도당에 반응하여 분비가 증가합니다. 인슐린 작용은 인슐린이 표적 세포막의 세포 표면 수용체와 결합하여 이루어집니다. 인슐린의 결함은 고인슐린혈증과 제2형 당뇨병의 원인입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Insulin B 항체 (C-12) 참고문헌:

  1. 인슐린 및 IGF-1 수용체 세포질 도메인의 차별적 신호 전달 가능성.  |  Lammers, R., et al. 1989. EMBO J. 8: 1369-75. PMID: 2548842
  2. 인슐린 작용의 분자 메커니즘.  |  Kahn, CR. 1985. Annu Rev Med. 36: 429-51. PMID: 2986528
  3. 초활성 단량체 des-[Phe(B25)] 인간 인슐린 돌연변이의 용액 구조: des-[Phe(B25)] 인슐린의 단량체화 및 네이티브 인슐린의 이량체화에 대한 구조적 근거 규명.  |  Jørgensen, AM., et al. 1996. J Mol Biol. 257: 684-99. PMID: 8648633
  4. 랑게르한스 췌장 섬에서 세포질 칼슘 진동과 인슐린 방출.  |  Soria, B. and Martin, F. 1998. Diabetes Metab. 24: 37-40. PMID: 9534007
  5. 프로인슐린: 최근의 관찰과 논란.  |  Mackin, RB. 1998. Cell Mol Life Sci. 54: 696-702. PMID: 9711235

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Insulin B 항체 (C-12)

sc-377071
200 µg/ml
$316.00

Insulin B (C-12): m-IgG Fc BP-HRP 번들

sc-529966
200 µg Ab; 10 µg BP
$354.00

Insulin B (C-12): m-IgGκ BP-HRP 번들

sc-523173
200 µg Ab, 40 µg BP
$354.00

Insulin B (C-12): m-IgG1 BP-HRP 번들

sc-543793
200 µg Ab; 20 µg BP
$354.00

Insulin B 항체 (C-12) AC

sc-377071 AC
500 µg/ml, 25% agarose
$416.00

Insulin B 항체 (C-12) HRP

sc-377071 HRP
200 µg/ml
$316.00

Insulin B 항체 (C-12) FITC

sc-377071 FITC
200 µg/ml
$330.00

Insulin B 항체 (C-12) PE

sc-377071 PE
200 µg/ml
$343.00

Insulin B 항체 (C-12) Alexa Fluor® 488

sc-377071 AF488
200 µg/ml
$357.00

Insulin B 항체 (C-12) Alexa Fluor® 546

sc-377071 AF546
200 µg/ml
$357.00

Insulin B 항체 (C-12) Alexa Fluor® 594

sc-377071 AF594
200 µg/ml
$357.00

Insulin B 항체 (C-12) Alexa Fluor® 647

sc-377071 AF647
200 µg/ml
$357.00

Insulin B 항체 (C-12) Alexa Fluor® 680

sc-377071 AF680
200 µg/ml
$357.00

Insulin B 항체 (C-12) Alexa Fluor® 790

sc-377071 AF790
200 µg/ml
$357.00

Insulin B 항체 (C-12) B

sc-377071 B
200 µg/ml
$320.00

Insulin B (C-12) 중화펩타이드

sc-377071 P
100 µg/0.5 ml
$68.00

The molucular weight of insulin is 5808 Da, why the target band of this antibody is about 20 Kda?

Asked by: Ling
Thank you for your question. Please contact technical service by email scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-09-25

Can I use SC-377071 for sandwich ELISA?

Asked by: marthatyas
Thank you for your question. This antibody, sc-377071 Insulin B (C-12), has not been tested for sandwich ELISA, but it is recommended and warrantied for direct or indirect ELISA.
Answered by: Tech Service
Date published: 2019-09-18

Have you test this antibody through Native-Page Western Blot? Thanks

Asked by: marthatyas
Thank you for your question. No, this antibody has not been tested for Native-Page Western blot.
Answered by: Tech Service
Date published: 2019-09-15

I have contacted SCBT south east asia branch roughly 2 weeks a go to enquire about the 10 ug sample free of charge as stated in the product information Insulin B (C-12): sc-377071. However, there is no response. Can you help me regarding this matter?

Asked by: Agung
We are sorry to hear that you have not yet received a reply. If you are currently located in Indonesia, please contact one of our authorized distributors for information about how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-27

The expected molecular weight of Insulin is 6-7 kDa. Why 12 kDa? It´s not clear. Thank you.

Asked by: Jimena
Thank you for your question. The predicted molecular weight of 12 kDa is for the precursor of Insulin B which is 110 amino acids in length.
Answered by: Technical Support
Date published: 2023-10-04

what's the molecular weight of insulin (c-12)?

Asked by: weitang
Thank you for your question. Insulin B Antibody (C-12): sc-377071 is recommended for detection of Insulin B chain of mouse, rat and human origin. The expected molecular weight of Insulin B is 12 kDa.
Answered by: Technical Support
Date published: 2017-11-06

How can Insulin B Antibody (C-12): sc-377071 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-377071 PE), fluorescein (sc-377071 FITC), Alexa Fluor® 488 (sc-377071 AF488) or Alexa Fluor® 647 (sc-377071 AF647).
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 5 out of 5 by from Excellent Signal!Just wanted to say how pleased I am with the antibody sample I was given! Great strong insulin signal on my mouse paraffin embedded sections for IF staining! Highly recommend!
Date published: 2017-09-13
Rated 4 out of 5 by from Primate retinaAntibody was tested under standard conditions (Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART). Peptides. 2016 Oct;84:22-35). The isolated retina of a baboon was fixed for 60 minutes in 4% paraformaldehyde. Tissue was incubated for 9 days in a 1:1,000 solution of primary antibody with detergent and a blocking agent at 4 °C, washed, then incubated in an affinity-purified secondary antibody for 4 days at 4 °C, washed, and mounted in medium containing DAPI. No peptide blocking control was performed. Results: Very light punctate labeling and low background throughout the optic fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer (less).
Date published: 2017-06-01
Rated 5 out of 5 by from greatIn our experiment, we use this product testing, this has been working really well
Date published: 2017-05-17
Rated 5 out of 5 by from High antibody titerget this antibody two months ago,the antibody titer was high while doing immunofluorescence(1:200)
Date published: 2017-05-04
Rated 5 out of 5 by from specific staningThe results insulin mainly localizes to cytoplasm of embryonic cardiomyocytes. Patchy staining was also observed in nuclei.
Date published: 2017-01-29
Rated 5 out of 5 by from Superb resultsStrong results for both western blot and ihc applications
Date published: 2017-01-03
Rated 5 out of 5 by from Good Insulin antibodyA good choice for detecting Insulin B by Western or IHC. It has great specificity and will provide clean blots and stains with strong signal.
Date published: 2016-12-29
Rated 4 out of 5 by from Worked wellGot this as a sample antibody to try out and it worked well on our mouse pancreatic cell line.
Date published: 2016-11-29
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Insulin B 항체 (C-12) is rated 4.8 out of 5 by 12.
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