IL-4I1 Double Nickase Plasmid (h): sc-415270-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-4I1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-4I1 Double Nickase Plasmid (h) and IL-4I1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL4I1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-4I1 Double Nickase Plasmid (h) | sc-415270-NIC | 20 µg | $410.00 | |||
IL-4I1 Double Nickase Plasmid (h2) | sc-415270-NIC-2 | 20 µg | $410.00 |
Human IL4I1 encodes IL-4I1, a secreted L-amino acid oxidase expressed primarily by antigen-presenting cells that modulates immune activation through amino acid catabolism and redox signaling. By oxidizing aromatic amino acids and generating hydrogen peroxide and downstream metabolites, IL-4I1 can influence T cell proliferation, cytokine programs, and antigen-driven immune synapse function. Its activity intersects with pathways governing inflammatory signaling, metabolic reprogramming, and oxidative stress responses in myeloid and lymphoid compartments. Altered IL4I1 expression has been reported in diverse immunopathology and tumor-immune contexts, making it relevant for mechanistic studies of immune regulation and microenvironmental control.
IL-4I1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL4I1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL4I1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL4I1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL4I1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.