IFRD2 CRISPR/Cas9 KO Plasmid (m): sc-421054
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFRD2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IFRD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFRD2 CRISPR/Cas9 KO Plasmid (m) | sc-421054 | 20 µg | $397.00 |
Overview
Interferon-related developmental regulator 2 (IFRD2) is a transcriptional coregulator implicated in controlling gene expression programs that shape cellular differentiation and stress-adaptive responses. In mouse systems, IFRD2 is studied for its potential roles in modulating chromatin-associated transcriptional complexes and coordinating context-dependent signaling outputs that influence proliferation and lineage commitment. Altered regulation of IFRD-family proteins has been linked to disrupted developmental processes and aberrant inflammatory or stress-response signaling, making Ifrd2 a useful locus for mechanistic studies in tissue homeostasis. Targeting Ifrd2 can help clarify how transcriptional repression/activation dynamics interface with downstream pathways governing cell fate and remodeling.
IFRD2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ifrd2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ifrd2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ifrd2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IFRD2 protein expression.
This CRISPR knockout system enables efficient generation of Ifrd2-deficient cell models for investigation of IFRD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ifrd2 exon(s) critical for IFRD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ifrd2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IFRD2 CRISPR/Cas9 KO Plasmid (m) and IFRD2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ifrd2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IFRD2 HDR Plasmid (m) and IFRD2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ifrd2 homology arms to support homology-directed repair at defined Ifrd2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.