Id2 항체 (E-7) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Id2을 검출할것을 권장합니다.

Id2 항체 (E-7): sc-398104

Id2 항체 (E-7) is rated 4.7 out of 5 by 7.
  • y_2021, m_3, d_5, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.14
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_ko_KR, sid_398104, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 128ms
  • REVIEWS, PRODUCT
5
5
4
2
3
0
2
0
1
0

데이터 시트

    • 안티-Id2 항체 E-7 는 마우스 monoclonal IgG2a Id2 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 mouse origin의 Id2의 내부에 위치한 105-134의 아미노산에 해당됩니다.
    • 안티-Id2 항체 (E-7)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 Id2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Id2 항체 (E-7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-398104 X, 200 µg/0.1 ml)이 있습니다.
    • Id2 (E-7): sc-398104무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for Id2 Antibody (E-7) for WB applications. This reagent is now offered in a bundle with Id2 Antibody (E-7) (see ordering information below).

can it used for pig?

Asked by: huarenwu
Thank you for your question. We have not yet tested this antibody, Id2 (E-7): sc-398104, for use in pig samples so we do not recommend it for use with this species at this time. The antibody is raised against amino acids 105-134 at the C-terminus of Id2 of mouse origin. Based on sequence analysis, this sequence shares 90% sequence identity with Id2 of pig origin (protein accession number Q2VIU1). This is a high degree of identity, so there is a good chance that the antibody may also cross-react with pig.
Answered by: Technical Support
Date published: 2017-09-13

What application is the blocking peptide sc-398104 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_3, d_5, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.14
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_ko_KR, sid_398104, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 130ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good antibody for Id2 detection by Western blot We tested this antibody on differentiating N1E-115 mouse cell line. It gives a clear signal at about 20 kDa, in line with the expected molecular weight of the protein (17,5 kDa). Background is very minimal. Antibody dilution: 1:250 in PBST with 5% nonfat dry milk.
Date published: 2021-02-17
Rated 4 out of 5 by from some background, but strong signal in WB diluted 1:100 in TBST, 5% BSA the AB detects endogenous and recombinant fusion protein in WB in NSCLC cell lines with some, yet weaker background
Date published: 2017-11-18
Rated 5 out of 5 by from Antibody effect is very good we are using renal tubular epithelial cells, with IF detection of Id2 expression, dilution ratio is 1: 80, the results are very good,Fluorescence is shown in the nucleus,antibody effect is very good.
Date published: 2017-10-12
Rated 5 out of 5 by from Great features Antibody effect is very good, we are using HT22 cells, with western blot detection of Id2 expression, dilution ratio is 1: 500, the results are very good.
Date published: 2017-07-17
Rated 5 out of 5 by from For therapy The Id2 antibody we used was mouse monoclonal IgG2a provided at 200 μg / ml, as suggested by WB, IP, IF, and ELISA as shown in the figure, and we performed a perfect procedure for targeted therapy
Date published: 2017-02-09
Rated 5 out of 5 by from Versatile and Clean Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-27
Rated 5 out of 5 by from Produced positive Western blot data of Id2 Produced positive Western blot data of Id2 expression in non-transfected and mouse Id2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-07
  • y_2021, m_3, d_5, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.14
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_ko_KR, sid_398104, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

산타크루즈바이오테크놀러지는 생물의학연구에 필요한 제품을 개발하는 선구자입니다. Toll Free 1-800-457-3801로 연락주세요. Copyright © 2007-2021 Santa Cruz Biotechnology, Inc. 판권소유. "Santa Cruz Biotechnology", 와 Santa Cruz Biotechnology, Inc. 로고, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", 및 San Juan Ranch 로고, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz"와 "EZ Touch" 는 Santa Cruz Biotechnology, Inc의 등록된 상표입니다. 모든 상표는 각자 소유주의 소유입니다.