HuD 항체 (H-9) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 HuD을 검출할것을 권장합니다.

HuD 항체 (H-9): sc-48421

HuD 항체 (H-9) is rated 5.0 out of 5 by 9.
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데이터 시트

    • 안티-HuD 항체 H-9 는 마우스 monoclonal IgG2a κ HuD 항체, 10간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 HuD에서 내부에 위치한 1-300 아미노산을 항원으로 사용하였습니다.
    • 안티-HuD 항체 (H-9)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 HuD 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-HuD 항체 (H-9)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • HuD (H-9): sc-48421무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for HuD Antibody (H-9) for WB and IHC(P) applications. These reagents are now offered in bundles with HuD Antibody (H-9) (see ordering information below).

HuD Antibody (H-9): sc-48421: Can you please provide the clone ID information?

Asked by: Leena Kumar
Thank you very much for your question. We produced this antibody against a recomb. protein fragment consisting of amino acids 1-300 of HuD of human origin. The same antigen was used for the other currently available antibody. https://www.scbt.com/scbt/browse/hud-Antibodies/_/N-11n1jcq The exact binding epitope for this monoclonal antibody has not been mapped, yet.
Answered by: Technical Support Europe
Date published: 2018-01-16

What is the total volume provided of HuD (H-9):sc-48421 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

Can HuD (H-9): sc-48421 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, HuD (H-9): sc-48421 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Strong signal Has good signal for paraffin-embedded sample for fluorescent immunohistochemistry.
Date published: 2017-05-16
Rated 5 out of 5 by from Superb for multiple applications !! Great IHC staining results; also strong WB; much better than polyclonal alternatives
Date published: 2017-01-19
Rated 5 out of 5 by from Great antibody for staining This HuD antibody shows good results for IHC in human tissue.
Date published: 2017-01-12
Rated 5 out of 5 by from great antibody I tried this as a sample antibody from Santa Cruz and it really worked well on mouse intestine cryo sections at 1/ 400 dilution for myenteric ganglia.
Date published: 2016-12-23
Rated 5 out of 5 by from Working well in IF in zebrafish Working well in IF in zebrafish.
Date published: 2016-08-08
Rated 5 out of 5 by from Were able to publish for use in IF in rat tissue Were able to publish for use in IF in rat tissue.
Date published: 2015-06-04
Rated 5 out of 5 by from Positive signal in WB in SK Positive signal in WB in SK-N-SH and SH-SY5Y whole cell lysates and mouse and rat brain tissue extracts. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclear Produced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-03-15
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