Date published: 2025-9-10

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His-Tag 항체 (AD1.1.10): sc-53073

4.8(23)
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데이터 시트
  • His-Tag 항체(AD1.1.10) 는 마우스 monoclonal IgG1κ, 66간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 6개의 히스태그(His-tag) 폴리펩티드에 대해 항체 생성
  • His-Tag 항체(AD1.1.10)는 WB, IP and IF으로 fusion proteins encoded by polyhistidine expression vectors 를 감지하는 데에 추천한다 .
  • 항-His-Tag 항체(AD1.1.10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • His-Tag (AD1.1.10): sc-53073무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP 는 His-Tag 항체 (AD1.1.10) for WB applications. 이 시약은 이제 His-Tag 항체 (AD1.1.10)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

더보기

히스타그린 항체(AD1.1.10)는 6x 히스타그린 태그 폴리펩티드에 대해 생성된 마우스 단일 클론 IgG1 카파 경쇄 항체로, 웨스턴 블랏팅(WB), 면역침전법(IP), 면역형광법(IF) 등의 응용을 위해 설계되었습니다. 히스태그는 유전자 공학 기술을 통해 관심 있는 단백질의 N-말단이나 C-말단에 추가되는 6개의 히스티딘 잔기들의 짧은 서열입니다. 이 태그는 재조합 단백질의 정제 및 검출을 용이하게 해주기 때문에 매우 중요합니다. 히스티딘 잔기들은 니켈이나 코발트 같은 금속 이온에 대한 친화력이 강하기 때문에, 친화성 크로마토그래피 과정에서 태그가 부착된 단백질이 금속 킬레이트화 수지와 효율적으로 결합할 수 있도록 해줍니다. 이 때문에 단 한 번의 단계로 정제 과정을 거칠 수 있으며, 이 과정은 매우 효율적이며 단백질의 고유한 구조와 기능을 보존할 수 있습니다. 히스태그의 작은 크기는 단백질 활동에 대한 간섭을 최소화하기 때문에 단백질 연구에 이상적인 선택입니다. 히스태그를 이해하고 활용하는 것은 단백질 발현, 구조-기능 분석, 치료제 개발에 종사하는 연구자들에게 중요합니다. 왜냐하면 히스태그를 활용하면 연구하기 어려운 단백질을 쉽게 분리하고 특성을 파악할 수 있기 때문입니다. 항히스타글루티닌 항체(AD1.1.10)는 히스타글루티닌 서열에 특이적으로 결합하기 때문에, 연구자들은 다양한 실험 응용 분야에서 히스타글루티닌 태그가 붙은 단백질을 검출하고 분석할 수 있으며, 따라서 분자생물학, 생화학 및 관련 분야의 연구가 발전할 수 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

His-Tag 항체 (AD1.1.10)

sc-53073
200 µg/ml
$316.00

His-Tag (AD1.1.10): m-IgG Fc BP-HRP 번들

sc-526762
200 µg Ab; 10 µg BP
$354.00

His-Tag (AD1.1.10): m-IgG1 BP-HRP 번들

sc-532135
200 µg Ab; 20 µg BP
$354.00

His-probe 항체 (AD1.1.10) AC

sc-53073 AC
500 µg/ml, 25% agarose
$416.00

His-probe 항체 (AD1.1.10) HRP

sc-53073 HRP
200 µg/ml
$316.00

His-probe 항체 (AD1.1.10) FITC

sc-53073 FITC
200 µg/ml
$330.00

His-probe 항체 (AD1.1.10) PE

sc-53073 PE
200 µg/ml
$343.00

His-probe 항체 (AD1.1.10) Alexa Fluor® 488

sc-53073 AF488
200 µg/ml
$357.00

His-probe 항체 (AD1.1.10) Alexa Fluor® 546

sc-53073 AF546
200 µg/ml
$357.00

His-probe 항체 (AD1.1.10) Alexa Fluor® 594

sc-53073 AF594
200 µg/ml
$357.00

His-probe 항체 (AD1.1.10) Alexa Fluor® 647

sc-53073 AF647
200 µg/ml
$357.00

His-probe 항체 (AD1.1.10) Alexa Fluor® 680

sc-53073 AF680
200 µg/ml
$357.00

His-probe 항체 (AD1.1.10) Alexa Fluor® 790

sc-53073 AF790
200 µg/ml
$357.00

Can this antibody detect the His-tag on the N- and/or C-terminus?

Asked by: Nick
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-08-06

Does this antibody detect the His-tag on both the N- and the C-terminus?

Asked by: Nick
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-08-06

This antibody can detect His tagged at the middle of the protein construct by Western Blot (while many products can't). 

Asked by: Pattama
Thank you for your question. Please contact our Technical Service Department by phone, e-mail, or live chat. We are happy to help you with any questions regarding this antibody.
Answered by: SCBT Representative
Date published: 2022-03-09

If i use the HIS antibody for an IP to pull-down a HIS-Tagged protein, could imidazole be used to elute the HIS tag protein from the HIS antibody ?

Asked by: j1234t1234
Thank you for your question. We have not tested the antibody using this type of elution buffer. Please refer to the product citations on our website using this antibody for IP experiments, or contact us for further Technical Support.
Answered by: Technical Support
Date published: 2023-04-23

Is His-probe Antibody (AD1.1.10): sc-53073 available to useful to FACS?

Asked by: piliya
Thank you for your question. We have not tested this antibody, His-probe (AD1.1.10): sc-53073, for FACS or FCM and so these are not recommended applications at this time.
Answered by: Technical Support
Date published: 2023-04-23

Is His-probe Antibody (AD1.1.10): sc-53073 available to useful to ELISA? If not, please recommend.

Asked by: George C
Thank you for your question. We are aware of one publication citing use of this antibody, His-probe (AD1.1.10): sc-53073, for use in an ELISA application. Please refer to PMID number 25174000 on PubMed for this citation. As an alternative, we would recommend using our mouse monoclonal antibody, His-probe (H-3): sc-8036, which is very popular and has been cited in over 10 publications for use in ELISA applications. https://www.scbt.com/scbt/product/his-probe-antibody-h-3
Answered by: Technical Support
Date published: 2023-04-23

Is His-probe Antibody (AD1.1.10): sc-53073 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using His-probe (H-3), which is available conjugated to agarose, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. (https://www.scbt.com/scbt/product/his-probe-antibody-h-3)
Answered by: Technical Support 8
Date published: 2023-11-25
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Rated 5 out of 5 by from The antibody is great!The antibody works very well and is highly recommended, I trust santa cruz!
Date published: 2025-05-27
Rated 5 out of 5 by from Excellent!I bought this product. It's very easy to use. I recommend you to buy this product.
Date published: 2024-01-11
Rated 5 out of 5 by from 功能很出色实验室一直都是用的santa的各种抗体,总的来说效果还不错 实验室一直都是用的santa的各种抗体,总的来说效果还不错
Date published: 2023-09-12
Rated 5 out of 5 by from 功能很出色实验室用的都是santa的抗体,总的来说效果还不错,还可以,实验室用的都是santa的抗体,总的来说效果还不错,还可以
Date published: 2023-09-12
Rated 5 out of 5 by from GhhhOke. Good. Skkwmmekdmmsksmsksksoksjsjsjsjsbhwuwhwhhsh
Date published: 2023-08-09
Rated 4 out of 5 by from YesPhotoshop verry good thank you app Verry,verry good
Date published: 2023-07-28
Rated 4 out of 5 by from Good antibody for ELISAAntibody works well in ELISA to detect His-tagged proteins. Low or no background and detection of native and denatured proteins possible.
Date published: 2023-01-27
Rated 5 out of 5 by from Work well for Western BlotThis antibody works well to detect HIS tagged at the middle of the protein construct (while some antibodies from other makers can't).
Date published: 2022-03-08
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His-Tag 항체 (AD1.1.10) is rated 4.8 out of 5 by 23.
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