HECTD3 CRISPR/Cas9 KO Plasmid (h): sc-406532
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HECTD3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HECTD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HECTD3 CRISPR/Cas9 KO Plasmid (h) | sc-406532 | 20 µg | $397.00 |
Overview
HECTD3 encodes a HECT-domain E3 ubiquitin ligase that catalyzes ubiquitin transfer to target proteins, shaping their stability, localization, and signaling output. It has been linked to regulation of innate immune and inflammatory signaling, including modulation of NF-κB- and interferon-associated pathways through ubiquitin-dependent control of pathway components. By tuning proteostasis and signal transduction, HECTD3 can influence cellular stress responses, proliferation, and apoptosis. Dysregulated ubiquitination programs involving HECTD3 have been associated with cancer-related phenotypes and immune-mediated disease mechanisms, supporting its use in pathway dissection models.
HECTD3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HECTD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HECTD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HECTD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HECTD3 protein expression.
This CRISPR knockout system enables efficient generation of HECTD3-deficient cell models for investigation of HECTD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HECTD3 exon(s) critical for HECTD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HECTD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HECTD3 CRISPR/Cas9 KO Plasmid (h) and HECTD3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HECTD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HECTD3 HDR Plasmid (h) and HECTD3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HECTD3 homology arms to support homology-directed repair at defined HECTD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.