HCR CRISPR/Cas9 KO Plasmid (h): sc-407960
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HCR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HCR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HCR Antibody (E-4): sc-365889
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HCR CRISPR/Cas9 KO Plasmid (h) | sc-407960 | 20 µg | $397.00 |
Overview
CCHCR1 encodes the human coiled-coil α-helical rod protein (HCR), a cytoplasmic and perinuclear protein implicated in regulation of keratinocyte differentiation, epidermal barrier homeostasis, and cytoskeletal organization. HCR has been linked to processes influencing vesicular trafficking and cell proliferation, consistent with its enrichment in skin and hair follicle biology. Genetic variation and altered expression of CCHCR1 are associated with susceptibility to psoriasis and related inflammatory skin phenotypes, supporting its relevance to pathways connecting epithelial stress responses and immune signaling. As a result, CCHCR1 is frequently studied in models of keratinocyte biology, inflammatory signaling crosstalk, and skin disease genetics.
HCR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCHCR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCHCR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCHCR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HCR protein expression.
This CRISPR knockout system enables efficient generation of CCHCR1-deficient cell models for investigation of HCR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCHCR1 exon(s) critical for HCR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCHCR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HCR CRISPR/Cas9 KO Plasmid (h) and HCR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCHCR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HCR HDR Plasmid (h) and HCR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCHCR1 homology arms to support homology-directed repair at defined CCHCR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.