HAPLN4 항체 (H-6) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HAPLN4을 검출할것을 권장합니다.

HAPLN4 항체 (H-6): sc-271450

HAPLN4 항체 (H-6) is rated 4.3 out of 5 by 3.
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데이터 시트

    • 안티-HAPLN4 항체 H-6 는 마우스 monoclonal IgG1 (kappa light chain) HAPLN4 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 HAPLN4에서 에 가까운229-273의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
    • 안티-HAPLN4 항체 (H-6)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 HAPLN4 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-HAPLN4 항체 (H-6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • HAPLN4 (H-6): sc-271450무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HAPLN4 Antibody (H-6) for WB applications. These reagents are now offered in bundles with HAPLN4 Antibody (H-6) (see ordering information below).
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I would like to use antibodies against HAPLN3 and HAPLN4 in mouse and human tissue for immunofluorescence. What do you suggest for using mouse antibodies in mouse tissue to avoid intense background?

Asked by: Eduardo Araujo
Thank you for your inquiry. To avoid background issues with mouse-on-mouse staining we recommend using conjugated primary antibodies, these would be available by Special Order ([email protected]). Another option would be to use our Mouse IgG Binding Protein products in place of conventional secondaries to reduce non-specific binding (https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-130b1ry). Otherwise there are commercial Mouse-On-Mouse (MOM) staining kits that are designed to reduce non-specific staining. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-07-03

What application is the blocking peptide sc-271450 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from HAPLN4 Rat Cortex I stained for HAPLN4 in rat cortex ON at 4C using 1:500 dilution. I would suggest using 1:100-1:200 dilution instead.
Date published: 2018-04-10
Rated 5 out of 5 by from Produced positive Western Blot data of HAPLN4 Produced positive Western Blot data of HAPLN4 expression in Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Nice Western Blot data of HAPLN4 expression Nice Western Blot data of HAPLN4 expression in 293T whole cell lysate. -SCBT QC
Date published: 2014-01-31
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