Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAI-1 CRISPR/Cas9 KO Plasmid (h): sc-404011
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAI-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HAI-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAI-1 Antibody (H-1): sc-137159
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAI-1 CRISPR/Cas9 KO Plasmid (h) | sc-404011 | 20 µg | $397.00 |
Overview
SPINT1 encodes hepatocyte growth factor activator inhibitor type 1 (HAI-1), a membrane-anchored Kunitz-type serine protease inhibitor that constrains pericellular proteolysis at epithelial surfaces. HAI-1 regulates the activity and maturation of proteases such as matriptase (ST14) and hepsin, thereby shaping extracellular matrix remodeling, epithelial barrier integrity, and growth factor signaling cascades including HGF/c-MET. Through these interactions, SPINT1 contributes to epithelial homeostasis, wound repair, and control of cell motility and invasion-related programs. Dysregulated SPINT1/HAI-1 balance has been linked to altered protease networks observed in epithelial pathologies, including inflammatory injury and cancer-associated remodeling.
HAI-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPINT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPINT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPINT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HAI-1 protein expression.
This CRISPR knockout system enables efficient generation of SPINT1-deficient cell models for investigation of HAI-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPINT1 exon(s) critical for HAI-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPINT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HAI-1 CRISPR/Cas9 KO Plasmid (h) and HAI-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPINT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HAI-1 HDR Plasmid (h) and HAI-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPINT1 homology arms to support homology-directed repair at defined SPINT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.