Date published: 2025-9-8

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HA-Tag 항체 (4C12): sc-57594

4.3(3)
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데이터 시트
  • HA-Tag 항체(4C12) 는 마우스 monoclonal IgG1κ, 6간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • ATCC 균주 13313의 전체 세포에 대해 양성 반응
  • WB, IP와 IF으로 proteins containing the HA tag 을 검출할것을 권장합니다.
  • See HA-Tag (F-7): sc-7392 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP 는 HA-Tag 항체 (4C12) for WB applications. 이 시약은 이제 HA-Tag 항체 (4C12)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

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박테리아, 곤충 및 포유류 숙주에서 진핵생물 유전자의 코딩 영역을 발현하기 위한 플라스미드 벡터가 일반적으로 사용되며, 이러한 발현 벡터는 진핵생물 표적 단백질과 표적 단백질의 정제 및 시각화를 돕기 위해 고안된 특수 영역으로 구성된 하이브리드 융합 단백질을 코딩하는 데 자주 사용됩니다. 예를 들어, pCDM8 발현 벡터 및 그 유도체는 표적 단백질과 인플루엔자 단백질 헤마글루티닌(HA)에서 유래한 11개 아미노산 펩타이드 사이의 융합을 코딩합니다. HA 에피토프 태그는 발현된 융합 단백질의 웨스턴 블로팅 및 면역 조직 화학적 위치 확인에 유용하며, HA 에피토프 태그에 대해 특이적으로 생성된 항체로 검사할 때 유용합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

HA-Tag 항체 (4C12)

sc-57594
200 µg/ml
$316.00

HA-Tag (4C12): m-IgG Fc BP-HRP 번들

sc-539062
200 µg Ab; 10 µg BP
$354.00

HA-Tag (4C12): m-IgG1 BP-HRP 번들

sc-541237
200 µg Ab; 20 µg BP
$354.00

What is the recommended secondary for HA-probe (4C12): sc-57594 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-03-07

Does anyone use goat-anti-mouse IgG-HRP conjugate(sc-2005), and successfully find a band? (I used 1:200 dilution of primary Ab and 1:5000 of secondary Ab.)

Asked by: FYT0429
Thank you for your question. We have successfully used this mouse monoclonal IgG1 primary antibody, HA-probe (4C12): sc-57594, together with goat anti-mouse IgG-HRP conjugated secondary antibody (sc-2005). We would expect a signal using the same dilutions that you have tested. Please contact our Technical Service Department and we would be happy to assist you in troubleshooting these antibodies. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat. We would also recommend trying our recombinant mouse IgGκ binding protein alternatives to this conventional secondary anti-mouse IgG reagents. The product number is m-IgGκ BP-HRP: sc-516102.
Answered by: Technical Support
Date published: 2016-12-02
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Rated 5 out of 5 by from Nice antibody for western blot detectionWe used this antibody for western blot expression. We got a perfect band at the correct molecular weight
Date published: 2017-01-31
Rated 4 out of 5 by from Nice specific single bands on the WBNice specific single bands on the WB. -SCBT QC
Date published: 2014-04-22
Rated 4 out of 5 by from We used this a few times and saw a strongWe used this a few times and saw a strong protein band, but there was some background. -SCBT QC
Date published: 2013-06-02
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HA-Tag 항체 (4C12) is rated 4.3 out of 5 by 3.
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