Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gas6 Double Nickase Plasmid (h): sc-400978-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gas6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gas6 Double Nickase Plasmid (h) and Gas6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GAS6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gas6 Antibody (A-9): sc-376087
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gas6 Double Nickase Plasmid (h) | sc-400978-NIC | 20 µg | $410.00 | |||
Gas6 Double Nickase Plasmid (h2) | sc-400978-NIC-2 | 20 µg | $410.00 |
Growth arrest–specific 6 (GAS6) encodes Gas6, a vitamin K–dependent secreted ligand for the TAM receptor tyrosine kinases AXL, MERTK, and TYRO3. Gas6–TAM signaling regulates efferocytosis, innate immune homeostasis, platelet function, and cell survival programs through pathways that include PI3K–AKT, MAPK/ERK, and NF-κB, with context-dependent effects on proliferation and migration. In many tissues, altered GAS6/AXL axis activity has been linked to inflammatory remodeling, fibrosis, and tumor-associated signaling, making GAS6 a frequent target in studies of microenvironmental crosstalk. As a secreted factor, Gas6 also serves as a tractable node for dissecting receptor-ligand dynamics and paracrine regulation in co-culture and conditioned-media models.
Gas6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GAS6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GAS6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GAS6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GAS6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.