Date published: 2025-9-10

001 800-1338-3838

SCBT Portrait Logo
Seach Input

GAPDH 항체 (H-12): sc-166574

4.4(16)
리뷰 쓰기질문하기

데이터 시트
  • GAPDH 항체(H-12) 는 마우스 monoclonal IgG2aκ, 277간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 95-112의 GAPDH의 human 기원의 아미노산을 나타내는 펩타이드에 대해 제기됨
  • GAPDH 항체 (H-12)는 WB, IP, IF, IHC(P) and ELISA으로mouse, rat, human and 유래의 GAPDH 를 감지하는 데에 추천한다 ; 이외에, canine, bovine, porcine and avian등 species와 반응할수 있습니다
  • 항-GAPDH 항체(H-12)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • GAPDH (H-12): sc-166574무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG2a BP-HRPm-IgGκ BP-HRP 는 GAPDH 항체 (H-12) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은GAPDH 항체(H-12) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

더보기

글리세랄데히드-3-포스페이트 탈수소효소(GAPDH)는 우라실 DNA 글리코실라제라고도 하며, 탄수화물 대사에서 중요한 에너지 생산 단계인 무기 인산염과 니코틴아마이드 아데닌 디뉴클레오티드(NAD)의 존재하에 글리세랄데히드-3-포스페이트의 가역적 산화 인산화를 촉매하는 효소입니다. GAPDH는 해당 작용에 필수적인 역할을 하는 것으로 오랫동안 인식되어 왔지만, GAPDH의 추가 기능으로는 우리실 DNA 글리코실라제로서의 역할, 전사 활성화, RNA 결합 및 핵 RNA 수출, DNA 복제 및 DNA 복구에 관여하는 것 등이 있습니다. 간암, 폐암, 전립선암에서 GAPDH의 발현이 상향 조절됩니다. GAPDH는 세포 사멸 중에 핵으로 이동합니다. β-아밀로이드 전구체, 헌팅틴 및 기타 삼중 반복 신경 장애 단백질을 포함하여 인간 신경 퇴행성 질환에 관여하는 신경 단백질과 GAPDH 복합체를 형성합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

GAPDH 항체 (H-12)

sc-166574
200 µg/ml
$316.00

GAPDH (H-12): m-IgGκ BP-HRP 번들

sc-521661
200 µg Ab, 40 µg BP
$354.00

GAPDH (H-12): m-IgG2a BP-HRP 번들

sc-546102
200 µg Ab; 10 µg BP
$354.00

GAPDH 항체 (H-12) AC

sc-166574 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 항체 (H-12) HRP

sc-166574 HRP
200 µg/ml
$316.00

GAPDH 항체 (H-12) FITC

sc-166574 FITC
200 µg/ml
$330.00

GAPDH 항체 (H-12) PE

sc-166574 PE
200 µg/ml
$343.00

GAPDH 항체 (H-12) Alexa Fluor® 488

sc-166574 AF488
200 µg/ml
$357.00

GAPDH 항체 (H-12) Alexa Fluor® 546

sc-166574 AF546
200 µg/ml
$357.00

GAPDH 항체 (H-12) Alexa Fluor® 594

sc-166574 AF594
200 µg/ml
$357.00

GAPDH 항체 (H-12) Alexa Fluor® 647

sc-166574 AF647
200 µg/ml
$357.00

GAPDH 항체 (H-12) Alexa Fluor® 680

sc-166574 AF680
200 µg/ml
$357.00

GAPDH 항체 (H-12) Alexa Fluor® 790

sc-166574 AF790
200 µg/ml
$357.00

GAPDH (H-12) 중화펩타이드

sc-166574 P
100 µg/0.5 ml
$68.00

What is the recommended dilution for GAPDH Antibody (H-12) in IHC applications?

Asked by: Trav11
We recommend a starting dilution of 1:50 in IHC applications.
Answered by: Tech Service 4
Date published: 2017-01-06

Does this antibody show cross-reactivity with E. coli GAPDH?

Asked by: EGAR
Thank you for your inquiry. The immunogen used to raise our GAPDH antibody sc-166574 shows low homology with the sequence for E.coli GAPDH, so we do not predict it will detect this protein and have no data to support reactivity.
Answered by: Technical Service
Date published: 2016-10-28
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_ko_KR, sid_166574, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 96ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from discreto40ug of protein were loaded in AnyKD precasted gel Electrophoresis was performed in TGS buffer at 100V Immunoblot was performed with Protran Nitrocellulose Membrane at 400mA for 90’ Membranes were incubated 1h in BSA 5% in TBS + Tween 0.1% (TBS-T) GAPDH antibody was diluted 1:1000 in TBS-T and membranes incubated o/n at 4°C Membranes were washed three times with TBS-T for 10’ Secondary antibody anti-mouse HRP conjugated (Bio-Rad 170-6516) was diluted 1:20000 in TBS-T and added to membranes for 1h at RT Signal was detected with ECL (GE GE RPN2209) in the dark room Exposition time 1’ 3’ 15’ In our experimental conditions the antibody worked well and the signal was detected at expected molecular weight
Date published: 2019-07-26
Rated 5 out of 5 by from good bandsThe first time it didn't work as well. But the second time we used a better ECL and it gave as pretty dark bands so it works properly.
Date published: 2019-04-01
Rated 5 out of 5 by from very good antibodyworks on WB in dilution 1:500 in 5%BSA on mouse brain tissue
Date published: 2018-02-15
Rated 3 out of 5 by from work nicelyIt works well on vole's brain tissue extraction for western blot.
Date published: 2017-11-28
Rated 4 out of 5 by from GREAT!GREAT! This antibody worked great on IP for CHIP assay.
Date published: 2017-10-16
Rated 5 out of 5 by from The antibody is perfect for western blot.our lab always uses the antibody in last 5 years. the antibody exhibits excellent specific and reactivites in the WB analysis.
Date published: 2017-07-27
Rated 5 out of 5 by from This project is perfect !This product makes me happy!Because this is the only primary antibody that very useful from my a lab.Other antibodies are really normal ,even no bands on the computer screen sometimes.So the antibody from santa cruz that lets me more confidently when i doing experiment.And the price of these antibodies is not very expensive.I will introduce it to my friends of other teams.
Date published: 2017-07-21
Rated 5 out of 5 by from great antibodyHighly express in mouse liver tissue, the effect is very good
Date published: 2017-06-02
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_ko_KR, sid_166574, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
GAPDH 항체 (H-12) is rated 4.4 out of 5 by 16.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_ko_KR, sid_166574, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT