Date published: 2025-9-8

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GAPDH 항체 (G-9): sc-365062

4.7(70)
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데이터 시트
  • GAPDH 항체(G-9) 는 마우스 monoclonal IgG1κ, 1,636간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 GAPDH의 1-335 아미노산을 항원으로 사용하였습니다.
  • 안티-GAPDH 항체 (G-9)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 GAPDH and GAPDH-2 를 감지하는 데에 추천한다.
  • 항-GAPDH 항체(G-9)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • GAPDH (G-9): sc-365062무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP는 GAPDH 항체 (G-9) WB 및 IHC(P) 애플리케이션용입니다. 이 시약은 이제 GAPDH 항체 (G-9)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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GAPDH 항체(G-9)는 마우스, 쥐 및 인간 유래의 GAPDH 및 GAPDH-2를 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 GAPDH 항체입니다. GAPDH 항체(G-9)는 비접합 항-GAPDH 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-GAPDH 항체 형태로도 사용할 수 있습니다. GAPDH 항체(G-9)는 우라실 DNA 글리코실라제라고도 하는 글리세랄데히드-3-인산염 탈수소효소(GAPDH)를 특이적으로 검출합니다. GAPDH 단백질은 탄수화물 대사에서 중요한 에너지 생성 단계인 무기 인산염과 니코틴아마이드 아데닌 디뉴클레오티드(NAD)의 존재 하에서 글리세랄데히드-3-포스페이트의 가역적 산화 인산화를 촉매하는 효소입니다. GAPDH는 해당 작용에 필수적인 역할을 하는 것으로 오랫동안 인식되어 왔지만, GAPDH의 추가 기능으로는 우리실 DNA 글리코실라아제 역할, 전사 활성화, RNA 결합 및 핵 RNA 수출, DNA 복제 및 DNA 복구에 관여하는 것 등이 있습니다. 간암, 폐암, 전립선암에서 GAPDH의 발현이 상향 조절되어 이러한 조직이 GAPDH 항체(G-9)의 양성 대조군으로 적합하다는 것을 시사합니다. GAPDH는 세포 사멸 중에 핵으로 이동합니다. 따라서 GAPDH 항체(G-9)에 의한 면역 염색은 세포질 또는 핵 국소화를 초래할 수 있습니다. β-아밀로이드 전구체, 헌팅틴 및 기타 삼중 반복 신경 장애 단백질을 포함하여 인간 신경 퇴행성 질환에 관여하는 신경 단백질과 GAPDH 복합체.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

GAPDH 항체 (G-9)

sc-365062
200 µg/ml
$316.00

GAPDH (G-9): m-IgGκ BP-HRP 번들

sc-522129
200 µg Ab, 40 µg BP
$354.00

GAPDH (G-9): m-IgG1 BP-HRP 번들

sc-552050
200 µg Ab; 20 µg BP
$354.00

GAPDH 항체 (G-9) AC

sc-365062 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 항체 (G-9) HRP

sc-365062 HRP
200 µg/ml
$316.00

GAPDH 항체 (G-9) FITC

sc-365062 FITC
200 µg/ml
$330.00

GAPDH 항체 (G-9) PE

sc-365062 PE
200 µg/ml
$343.00

GAPDH 항체 (G-9) Alexa Fluor® 488

sc-365062 AF488
200 µg/ml
$357.00

GAPDH 항체 (G-9) Alexa Fluor® 546

sc-365062 AF546
200 µg/ml
$357.00

GAPDH 항체 (G-9) Alexa Fluor® 594

sc-365062 AF594
200 µg/ml
$357.00

GAPDH 항체 (G-9) Alexa Fluor® 647

sc-365062 AF647
200 µg/ml
$357.00

GAPDH 항체 (G-9) Alexa Fluor® 680

sc-365062 AF680
200 µg/ml
$357.00

GAPDH 항체 (G-9) Alexa Fluor® 790

sc-365062 AF790
200 µg/ml
$357.00

We are using this antibody on mouse brain samples. We are getting a huge band at around 50kDa along with GAPDH. How can we use this antibody to get only the specific band of GAPDH?

Asked by: Shashank
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service . Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-17

这款产品二抗用哪个m-igg1还是iggk

Asked by: djxk
感谢您的提问。GAPDH (G-9) 抗体在WB 和 IHC(P) 方面的应用,推荐的二抗检测试剂为m-IgGκ BP-HRP。 Thank you for your question. m-IgGκ BP-HRP is the preferred secondary detection reagent for GAPDH Antibody (G-9) for WB and IHC(P) applications.
Answered by: Technical Service SH
Date published: 2023-10-13

Hello! I am using the antibody GAPDH (G-9) Alexa Fluor 647 (sc-365062) for Immunofluorescence. Could you please share the protocol you used for Immunofluoresnce with cultured cells (not tissue samples). Thank you!

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-10-13

why I get two clearly bands around ~55KD and ~40KD in my results,which one is specific to my antibody?thank you

Asked by: kangxin
Thank you for your question. The expected molecular weight for GAPDH is around 37-38kDa, so the band around 40kDa is your expected band.
Answered by: Tech Service
Date published: 2019-12-11

Hi, My western results detecting GAPDH around 30ish. Is it non-specific band? Thanks

Asked by: Uzma
Thank you for your question. The expected molecular weight of GapDH is 37kDa, but it can appear a little higher or lower depending on factors such as gel, markers etc. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2023-10-13

Hi, I have used SCBT Abs a lot. Can you share buffer composition that Abs are in? DTT or reducing agent is quite important for our experiment Thanks

Asked by: DrBio
Thank you for your question. GAPDH (G-9): sc-365062 contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support
Date published: 2023-10-13

How does GAPDH compare to Actin as a protein loading control for time course studies?

Asked by: Professor Griffin
Thank you for your question. Both Actin and GAPDH are generally acceptable as loading controls for time course, RNAi and covalent mod studies. However, their molecular weights are slightly different; Actin (43kda), GAPDH (37kda). In addition, GAPDH is more relevant for non-differentiated lymphocytes, whereas Actin or GAPDH may be suitable for adherents.
Answered by: Technical Support
Date published: 2024-06-18
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Rated 4 out of 5 by from GAPDH Antibody for Cytosolic FractionsThe GAPDH antibody was instrumental in defining cytosolic fractions, and the enriched, well-defined band unequivocally demonstrated its exclusive presence in this fraction. This antibody's specificity significantly contributed to the accuracy of our cytosolic protein analyses.
Date published: 2024-01-04
Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaLo utilicé en una línea celular de mosquito (C6/36) y hubo un buen reconocimiento de la proteína en WB
Date published: 2023-10-09
Rated 5 out of 5 by from GAPDH antibodyanti-glyceraldehyde 3-phosphate dehydrogenase antibody used to probe membrane and aid in monitoring protein expressions.
Date published: 2023-06-30
Rated 5 out of 5 by from a very satisfying antibodyThis antibody works well, the protein bands are very clear and easily to determine. I'm very grateful for the scientific work that has been done with this antibody.
Date published: 2023-02-07
Rated 5 out of 5 by from Worked wellWe used this antibody in brain and peripheral tissues and it worked well.
Date published: 2023-01-06
Rated 5 out of 5 by from Very good antibodyWe have been using this antibody for long time. Its very nice
Date published: 2022-06-27
Rated 5 out of 5 by from Free fatty acid receptor 2This antibody is very good.I want to use it again.
Date published: 2022-05-19
Rated 5 out of 5 by from Nice clear bandsWe use this antibody regularly in my laboratory and it gives nice clear bands, particulalry sharp when doing 12% gels. You can use this at 1:5000.
Date published: 2022-04-07
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GAPDH 항체 (G-9) is rated 4.7 out of 5 by 70.
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