Date published: 2025-9-10
Santa Cruz 동물 건강 방문하기

001 800-1338-3838

SCBT Portrait Logo
Seach Input
연락하기
사인/가입 계정 번호
빠른 주문
장바구니 Items (0)
GAL4 [DBD] Antibody (RK5C1) - Western Blotting - Image 5673
GAL4 (DBD 1-147) 태그가 부착된 융합 단백질 (20 ng)의 서던 블롯 분석. 테스트된 항체에는 GAL4 [DBD] 항체 (RK5C1): sc-510은 1.0 µg/ml (A), 0.1 µg/ml (B) 및 0.01 µg/ml (C)에서 테스트되었으며, GAL4 (DBD): sc-577은 0.1 µg/ml (D) 및 0.01 µg/ml (E)에서 테스트되었습니다.
GAL4 (DBD 1-147) 태그가 부착된 융합 단백질 (20 ng)의 서던 블롯 분석. 테스트된 항체에는 GAL4 [DBD] 항체 (RK5C1): sc-510은 1.0 µg/ml (A), 0.1 µg/ml (B) 및 0.01 µg/ml (C)에서 테스트되었으며, GAL4 (DBD): sc-577은 0.1 µg/ml (D) 및 0.01 µg/ml (E)에서 테스트되었습니다.

GAL4 [DBD] 항체 (RK5C1): sc-510

4.5(11)
리뷰 쓰기질문하기

데이터 시트
  • GAL4 [DBD] 항체 RK5C1 는 마우스 monoclonal IgG2a κ GAL4 [DBD] 항체, 466간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노산 94-147에 대해 GAL4 의 항원 형성
  • GAL4 [DBD] 항체(RK5C1)는 WB and IP으로 GAL4 DNA binding domain 를 감지하는 데에 추천한다 .
  • 항-GAL4 [DBD] 항체(RK5C1)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • WB, IHC(P) 와 ELISA에 사용가능한 biotin결합제품 이 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-510 X, 200 µg/0.1 ml)이 있습니다.
  • GAL4 [DBD] (RK5C1): sc-510의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • GAL4 [DBD] 항체 (RK5C1) WB 애플리케이션용입니다. 이 시약은 현재 GAL4 [DBD] 항체 (RK5C1)(아래 주문 정보 참조)와 함께 번들로 제공됩니다.

빠른 링크

보조 제품
설명
데이터 시트 및 프로토콜
연구 정보

더보기

다음을 포함한 추가 GAL4 항체 GAL4-TA

GAL4 [DBD] 항체(RK5C1)는 웨스턴 블롯(WB)과 면역침전법(IP)을 통해 GAL4를 검출하는 마우스 단일클론 IgG2a 카파 경쇄 항체입니다. Anti-GAL4 항체(RK5C1)는 비접합형과 아가로스, 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합형 형태로 제공됩니다. 사카로마이세스 세레비시아(Saccharomyces cerevisiae)에서 유래된 GAL4는 중요한 전사 활성화제 역할을 하며 유전자 발현 조절에 중요한 역할을 합니다. N-말단 147개의 아미노산 잔기는 특이적이고 강력한 DNA 결합에 필수적이지만, 이러한 잔기들은 독립적으로 효율적인 전사 활성화를 촉진하지는 않습니다. 이러한 특징 때문에 GAL4는 분자 생물학에서 특히 GAL4를 다양한 전사 조절 인자의 활성화 영역과 결합하는 융합 단백질을 만드는 데 유용한 도구로 사용되고 있습니다. 이러한 융합은 효모, 식물, 곤충, 포유류 세포를 포함한 다양한 유기체의 전사를 효과적으로 활성화할 수 있습니다. 또한, GAL4는 연구자들이 특정 단백질-단백질 상호작용을 탐구할 수 있게 해줌으로써 세포 과정과 조절 메커니즘에 대한 이해를 향상시키는 데 중요한 역할을 하는 "2중 하이브리드" 분석법을 개발하는 데 중요한 역할을 했습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

GAL4 [DBD] 항체 (RK5C1) 참고문헌:

  1. 효모 또는 포유류 세포에서의 발현을 위한 GAL4 융합 벡터.  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
  2. 두 가지 하이브리드 시스템: 관심 단백질과 상호작용하는 단백질의 유전자를 식별하고 복제하는 방법입니다.  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
  3. 효모 STE12 단백질의 페로몬 의존적 인산화는 전사 활성화와 관련이 있습니다.  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
  4. 활성화 인자와 표적.  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
  5. 단백질과 단백질의 상호작용을 감지하는 새로운 유전자 시스템.  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
  6. 곰팡이 유전자 조절 메커니즘의 모델: 사카로미세스 세레비지애의 GAL 유전자.  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
  7. GAL4의 결실 분석은 두 개의 전사 활성화 세그먼트를 정의합니다.  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647

주문정보

제품명카탈로그 번호 단위가격수량관심품목

GAL4 [DBD] 항체 (RK5C1)

sc-510
200 µg/ml
$316.00

GAL4 [DBD] (RK5C1): m-IgG Fc BP-HRP 번들

sc-525422
200 µg Ab; 10 µg BP
$354.00

GAL4 [DBD] 항체 (RK5C1) X

sc-510 X
200 µg/0.1 ml
$316.00

GAL4 [DBD] 항체 (RK5C1) AC

sc-510 AC
500 µg/ml, 25% agarose
$416.00

GAL4 [DBD] 항체 (RK5C1) HRP

sc-510 HRP
200 µg/ml
$316.00

GAL4 [DBD] 항체 (RK5C1) FITC

sc-510 FITC
200 µg/ml
$330.00

GAL4 [DBD] 항체 (RK5C1) PE

sc-510 PE
200 µg/ml
$343.00

GAL4 [DBD] 항체 (RK5C1) Alexa Fluor® 488

sc-510 AF488
200 µg/ml
$357.00

GAL4 [DBD] 항체 (RK5C1) Alexa Fluor® 546

sc-510 AF546
200 µg/ml
$357.00

GAL4 [DBD] 항체 (RK5C1) Alexa Fluor® 594

sc-510 AF594
200 µg/ml
$357.00

GAL4 [DBD] 항체 (RK5C1) Alexa Fluor® 647

sc-510 AF647
200 µg/ml
$357.00

GAL4 [DBD] 항체 (RK5C1) Alexa Fluor® 680

sc-510 AF680
200 µg/ml
$357.00

GAL4 [DBD] 항체 (RK5C1) Alexa Fluor® 790

sc-510 AF790
200 µg/ml
$357.00

GAL4 [DBD] 항체 (RK5C1) B

sc-510 B
200 µg/ml
$320.00

Does this antibody work for flow cytometry?

Asked by: ABLab
Thank you for your question. sc-510 GAL4 [DBD] Antibody (RK5C1) can be used for Flow cytometry,WB, IP,IF and IHC. Here is our flow cytometry protocol : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-10-04

Hello. I would like to test a free sample of this antibody. Would you mind telling me how to proceed? Yours

Asked by: bfav
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-12-16

Does this antibody work in ICC/IHC?

Asked by: sciencebeetle
Thank you for your question. We have not yet tested this antibody for IF or IHC(P) and so these are not recommended applications at this time. However, we are aware of almost 20 different publications that cite use of the antibody for IF or IHC so we can extend our warranty to cover this application. Please contact Technical Service if you would like a list of the specific product citations, or use the product citation search on our website and you can search by application, species and antibody.
Answered by: Technical Support
Date published: 2018-12-14

Hello, Would it be possible to receive a free sample of this antibody?

Asked by: YuliaLampi
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Dear Sir/Madam, I would like to order a Gal4 antibody for immunohistochemistry in plant protoplast extracts. Would it be possible to receive a free sample of GAL4 (RK5C1): sc-510 to test it before buying it?

Asked by: Dmitri
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Dear Sir/Madam, I would like to order a Gal4 antibody for immunohistochemistry in zebrafish embryos. Would it be possible to receive a free sample of GAL4 (RK5C1): sc-510 to test it before buying it? Many thanks, Florence GIGER, PhD.

Asked by: FloG
Thank you for the question. Yes, we will contact you by email to provide a FREE 10 µg sample of GAL4 (RK5C1): sc-510.
Answered by: Technical Support Europe
Date published: 2017-12-27

I am using this antibody to try to detect yeast two hybrid Gal4DBD-fusion protein expression and I have not been able to detect any even with a high copy vector. What is the WB protocol used for this antibody? Dissolved in BSA or Milk? What concentration?

Asked by: aloha
Thank you for your question. We recommend a starting dilution of 1:200 with this antibody, and we recommend diluting in 5% milk with TBS-T. Here is a link to the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-12-08

Does this antibody work for Drosophila UAS/Gal4 system? Has anyone tried it?

Asked by: sameerbau
Thank you for your question. This monoclonal antibody, GAL4 (RK5C1): sc-510, is recommended for detection of GAL4 DNA binding domain, and is not species-specific. We are aware of several publications specifically using the antibody in a drosophila model. Please refer to the following publications on NCBI's PubMed as a reference, PMID's: 12819212, 10766736 and 12514098.
Answered by: Technical Support
Date published: 2017-06-27
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_9
  • loc_ko_KR, sid_510, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 133ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good antibody for immunoprecipitationI used this antibody for immunoprecipitation assay of transfected 293T cells and it worked very well. For 0.5 ml lysates I used 4 ul of antibody.
Date published: 2022-11-06
Rated 4 out of 5 by from good antibodyI use 1:200 in HEK293T transfected with GAL4 plasmid. It works very well.
Date published: 2020-08-25
Rated 5 out of 5 by from Gal4 antibody that works!we tried a free sample of this antibody since our invitrogen one did not seem to work on cell lysates
Date published: 2018-02-16
Rated 5 out of 5 by from Works both on WB and IPI have used this antibody before as agarose conjugate but this was my first time using it for both IP and WB. It works very well without any background. For WB I used it at 1:2000 in TBS O/N and for IP I used 2 ug for 250 ug of nuclear extracts.
Date published: 2017-08-27
Rated 5 out of 5 by from Excellent choiceHighly reliable antibody that is well cited in literature and performs cleanly, especially in Western Blot.
Date published: 2017-01-12
Rated 5 out of 5 by from Able to publish using the antibody in WBAble to publish using the antibody in WB in HEK293 cells and mouse 3T3-L1 cells.
Date published: 2015-09-23
Rated 4 out of 5 by from Strong band in WB and IHC in frican green monkeyStrong band in WB and IHC in frican green monkey, mouse, human COS-7, L cells, P19, HEK 293, 293T. -SCBT Publication Review
Date published: 2015-04-11
Rated 4 out of 5 by from Robust signal in WB in yeast recombinantRobust signal in WB in yeast recombinant GAL4 fusion protein. -SCBT QC
Date published: 2015-03-30
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_ko_KR, sid_510, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
GAL4 [DBD] 항체 (RK5C1) is rated 4.5 out of 5 by 11.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_ko_KR, sid_510, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 119ms
  • REVIEWS, PRODUCT
001 800-1338-3838
방문 Santa Cruz Animal Health|방문 San Juan Ranch
산타크루즈바이오테크놀러지는 생물의학연구에 필요한 제품을 개발하는 선구자입니다. Toll Free 1-800-457-3801로 연락주세요. Copyright © 2007-2025 Santa Cruz Biotechnology, Inc. 판권소유. “Santa Cruz Biotechnology”, 와 Santa Cruz Biotechnology, Inc. 로고, “Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, 및 San Juan Ranch 로고, “UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”와 “EZ Touch” 는 Santa Cruz Biotechnology, Inc 의 등록된 상표입니다. 모든 상표는 각자 소유주의 소유입니다.