G530011O06Rik CRISPR/Cas9 KO Plasmid (m): sc-437289
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G530011O06Rik CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the G530011O06Rik genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G530011O06Rik CRISPR/Cas9 KO Plasmid (m) | sc-437289 | 20 µg | $397.00 |
Overview
Mouse G530011O06Rik encodes a largely uncharacterized protein with limited functional annotation, making it a useful target for discovery-oriented studies of gene regulation and cellular homeostasis. Available evidence from genomic context and expression profiling suggests potential roles in fundamental processes such as transcriptional control, RNA metabolism, or intracellular signaling, depending on cell type and developmental stage. Because many RIKEN cDNA genes map to conserved mammalian loci, perturbation of G530011O06Rik can help clarify how poorly annotated proteins contribute to pathway architecture and phenotype. Systematic loss-of-function interrogation may be informative for identifying links to stress responses, differentiation programs, or disease-relevant molecular signatures in mouse model systems.
G530011O06Rik CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the G530011O06Rik gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the G530011O06Rik together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the G530011O06Rik open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish G530011O06Rik protein expression.
This CRISPR knockout system enables efficient generation of G530011O06Rik-deficient cell models for investigation of G530011O06Rik signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting G530011O06Rik exon(s) critical for G530011O06Rik function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple G530011O06Rik genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by G530011O06Rik CRISPR/Cas9 KO Plasmid (m) and G530011O06Rik CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the G530011O06Rik locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by G530011O06Rik HDR Plasmid (m) and G530011O06Rik HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by G530011O06Rik homology arms to support homology-directed repair at defined G530011O06Rik target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.