Date published: 2025-9-6

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Fra1 항체 (D-3): sc-376148

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데이터 시트
  • Fra1 항체 D-3 는 마우스 monoclonal IgG1 κ Fra1 항체, 23간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 Fra-1의 내부에 위치한 2-29의 아미노산에 해당됩니다.
  • Fra1 항체 (D-3)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Fra-1 를 감지하는 데에 추천한다.
  • 항-Fra1 항체(D-3)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-376148 X, 200 µg/0.1 ml)이 있습니다.
  • Fra1 (D-3): sc-376148무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 Fra1 항체 (D-3) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Fra1 항체(D-3) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

더보기

Fra-1 항체(D-3)는 마우스, 쥐 및 인간 유래의 Fra-1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 Fra-1 항체(FRA1 항체 또는 FOSL1 항체로도 지정됨)입니다. Fra-1 항체(D-3)는 비접합 항-Fra-1 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-Fra-1 항체 모두로 제공됩니다. v-Fos 종양 유전자는 처음에 두 개의 독립적인 쥐 골육종 바이러스 분리체와 조류 신세포종 바이러스에서 발견되었습니다. c-Fos, Fos B, Fra-1 및 Fra-2를 포함한 c-Fos 유전자 계열의 구성원은 다양한 약제에 의해 신속하고 일시적으로 유도되는 핵 인산화 단백질을 암호화하며 여러 유전자의 전사 조절자 역할을 합니다. 특정 DNA 반응 요소에 결합하는 동이합체 및 이합체를 형성하는 c-Jun 단백질과 달리, c-Fos 단백질은 Jun 유전자 계열의 구성원들과만 이합체로 활성화됩니다. 또한, 선택된 ATF/CREB 패밀리 멤버는 Fos 및 Jun과 함께 류신 지퍼 이량체를 형성할 수 있습니다. 서로 다른 이량체는 AP-1 및 CRE 부위에 대한 차별적인 특이성과 친화성을 나타냅니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Fra1 항체 (D-3)

sc-376148
200 µg/ml
$316.00

Fra1 (D-3): m-IgG Fc BP-HRP 번들

sc-529826
200 µg Ab; 10 µg BP
$354.00

Fra1 (D-3): m-IgGκ BP-HRP 번들

sc-522853
200 µg Ab, 40 µg BP
$354.00

Fra1 (D-3): m-IgG1 BP-HRP 번들

sc-543709
200 µg Ab; 20 µg BP
$354.00

Fra1 항체 (D-3) X

sc-376148 X
200 µg/0.1 ml
$316.00

Fra1 항체 (D-3) AC

sc-376148 AC
500 µg/ml, 25% agarose
$416.00

Fra1 항체 (D-3) HRP

sc-376148 HRP
200 µg/ml
$316.00

Fra1 항체 (D-3) FITC

sc-376148 FITC
200 µg/ml
$330.00

Fra1 항체 (D-3) PE

sc-376148 PE
200 µg/ml
$343.00

Fra1 항체 (D-3) Alexa Fluor® 488

sc-376148 AF488
200 µg/ml
$357.00

Fra1 항체 (D-3) Alexa Fluor® 546

sc-376148 AF546
200 µg/ml
$357.00

Fra1 항체 (D-3) Alexa Fluor® 594

sc-376148 AF594
200 µg/ml
$357.00

Fra1 항체 (D-3) Alexa Fluor® 647

sc-376148 AF647
200 µg/ml
$357.00

Fra1 항체 (D-3) Alexa Fluor® 680

sc-376148 AF680
200 µg/ml
$357.00

Fra1 항체 (D-3) Alexa Fluor® 790

sc-376148 AF790
200 µg/ml
$357.00

Fra1 (D-3) 차단 펩티드

sc-376148 P
100 µg/0.5 ml
$68.00

for western blot, what's the concentration did you use in the Hela cells shown in the datashe et? Thanks

Asked by: picia67
Thank you for your question. For Western Blotting experiments with this antibody we recommend a starting dilution of 1:100 and a dilution range of 1:100-1:1000.
Answered by: Tech Support Europe
Date published: 2021-02-18

Hello, I would like to use this antibody for ChIP. What dilution would you recommend? Do you have any validation data on the ChIP performance on this antibody?

Asked by: HesAmenya
Thank you for your question. This antibody has not yet been tested for ChIP, and we are not yet aware of any product citations using this particular antibody for any ChIP applications. We do extend our warranty to cover that research application for our TransCruz reagent for Gel Supershift and ChIP applications (sc-376148 X, 200 µg/0.1 ml). We currently offer a different antibody that has been published for use in ChIP applications. Please refer to our Fra-1 Antibody (C-12): sc-28310. And PMID's: 22198213 and 21088499.
Answered by: Technical Support
Date published: 2020-07-23

Welche Proteinmenge eignet sich am besten für einen Western Blot mit dem Fra-1 Antikörper?

Asked by: Neslihan
Vielen Dank für Ihre Frage. Die optimale Proteinmenge ist abhängig von dem verwendeten Sample. Generell empfehlen wir pro Bahn bei der Elektrophorese eine Proteinmenge von 40–60 µg bei Zelllysaten, 10–20 µg bei Kernextrakten, 5-10 µg bei transfizierten Zelllysaten, oder 10–20 ng bei aufgereinigtem Protein. What amount of protein is ideal for a western blot with Fra-1 Antibody (D-3): sc-376148? Thank you for your question. The ideal amount of protein is dependent on the sample that is being tested. We generally recommend 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

I would like to use Fra-1 Antibody (D-3): sc-376148 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376148 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-31
  • y_2025, m_8, d_28, h_5CST
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Rated 5 out of 5 by from good qualityreceived the samples weeks ago. blotting showed very strong signal
Date published: 2018-02-19
Rated 3 out of 5 by from Average featureswork well in WB at 1:500,Ges-1 Ccell,but when I recycle to use it,the titer is too inefficient,which led to a poor result.
Date published: 2017-12-05
Rated 5 out of 5 by from Works well for western blotThe antibody produced a good western blot for human tissue samples
Date published: 2016-12-19
Rated 5 out of 5 by from Positive detection of endogenously expressed FraPositive detection of endogenously expressed Fra-1 protein in NIH/3T3 cells (nuclear extracts used) through analysis by Western Blot. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Excellent immunoperoxidase nuclear and cytoplasmicExcellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Fra-1 protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-04
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Fra1 항체 (D-3) is rated 4.7 out of 5 by 6.
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