Date published: 2025-9-6
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FOXM1 Antibody (G-5) - Western Blotting - Image 353178
FOXM1 항체(G-5): sc-376471. PC-12 (A), NIH/3T3 (B), U-2 OS (C), Caco-2 (D), F9 (E) 및 MCF7 (F) 전체 세포 용해물에서 FOXM1 발현의 웨스턴 블롯 분석.
FOXM1 항체(G-5): sc-376471. PC-12 (A), NIH/3T3 (B), U-2 OS (C), Caco-2 (D), F9 (E) 및 MCF7 (F) 전체 세포 용해물에서 FOXM1 발현의 웨스턴 블롯 분석.

FOXM1 항체 (G-5): sc-376471

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데이터 시트
  • FOXM1 항체 G-5 는 마우스 monoclonal IgG2b κ FOXM1 항체, 64간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 FOXM1의 내부에 위치한 734-763의 아미노산에 해당됩니다.
  • FOXM1 항체 (G-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 all isoforms of FOXM1 를 감지하는 데에 추천한다.
  • 항-FOXM1 항체(G-5)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-376471 X, 200 µg/0.1 ml)이 있습니다.
  • FOXM1 (G-5): sc-376471의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 와 m-IgGκ BP-HRP 는 FOXM1항체 (G-5) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은FOXM1 항체(G-5) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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추가 FOX Antibodies 포함 FOXC1, FOXC2, FOXD1, FOXD3, FOXE3, FOXF2, FOXJ1, FOXJ2, FOXK1, FOXL1, FOXL2, FOXM1, FOXN1, FOXN2, FOXN4 및 FOXP1

FOXM1 항체(G-5)는 마우스, 쥐 및 인간 유래의 FOXM1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2b κ 마우스 모노클로날 FOXM1 항체(HNF3 항체, FKHL16 항체, 포크 헤드 박스 M1 항체로도 지정됨)입니다. FOXM1 항체(G-5)는 비공액형 항 FOXM1 항체 형태와 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 컨쥬게이트 등 다양한 공액형 항 FOXM1 항체 형태로 제공됩니다. Fox 전사 인자 계열은 날개나선 또는 포크헤드 도메인이라고 하는 공통 DNA 결합 도메인을 공유하는 대규모 단백질 그룹입니다. FKHL16, MPP2 또는 트라이던트로도 알려진 FOXM1은 주로 증식하는 세포에서 발현됩니다. 인간 FOXM1을 암호화하는 유전자는 염색체 12p13에 매핑됩니다. 증식하는 세포에서 FOXM1 발현을 제한하는 전사 요소는 시작 코돈의 300bp 상류에 위치합니다. FOXM1은 흉선, 고환, 소장 및 결장에 가장 풍부합니다. 대체 스플라이싱은 빠른 단백질 분해에 관여하는 PEST 영역을 포함하는 FOXM1A 및 FOXM1B 이소폼을 생성합니다. FOXM1 발현의 감소는 세포 증식의 노화 관련 결함과 관련이 있습니다. 반대로, 나이가 든 형질전환 생쥐의 간에서 FOXM1B 발현이 증가하면 간세포 DNA 복제 속도가 젊은 간에서 나타나는 높은 속도로 회복됩니다. FOXM1B는 사이클린 B1, 사이클린 D1 및 Cdc25B의 전사를 활성화합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

FOXM1 항체 (G-5)

sc-376471
200 µg/ml
$316.00

FOXM1 (G-5): m-IgG Fc BP-HRP 번들

sc-529873
200 µg Ab; 10 µg BP
$354.00

FOXM1 (G-5): m-IgGκ BP-HRP 번들

sc-522963
200 µg Ab, 40 µg BP
$354.00

FOXM1 (G-5): m-IgG2b BP-HRP 번들

sc-550146
200 µg Ab; 10 µg BP
$354.00

FOXM1 항체 (G-5) X

sc-376471 X
200 µg/0.1 ml
$316.00

FOXM1 항체 (G-5) AC

sc-376471 AC
500 µg/ml, 25% agarose
$416.00

FOXM1 항체 (G-5) HRP

sc-376471 HRP
200 µg/ml
$316.00

FOXM1 항체 (G-5) FITC

sc-376471 FITC
200 µg/ml
$330.00

FOXM1 항체 (G-5) PE

sc-376471 PE
200 µg/ml
$343.00

FOXM1 항체 (G-5) Alexa Fluor® 488

sc-376471 AF488
200 µg/ml
$357.00

FOXM1 항체 (G-5) Alexa Fluor® 546

sc-376471 AF546
200 µg/ml
$357.00

FOXM1 항체 (G-5) Alexa Fluor® 594

sc-376471 AF594
200 µg/ml
$357.00

FOXM1 항체 (G-5) Alexa Fluor® 647

sc-376471 AF647
200 µg/ml
$357.00

FOXM1 항체 (G-5) Alexa Fluor® 680

sc-376471 AF680
200 µg/ml
$357.00

FOXM1 항체 (G-5) Alexa Fluor® 790

sc-376471 AF790
200 µg/ml
$357.00

FOXM1 (G-5) 중화펩타이드

sc-376471 P
100 µg/0.5 ml
$68.00

FOXM1 G-5 is mouse monoclonal IgG2b. I would like to know if it can be used with conventional secondary anti-mouse IgG reagents.

Asked by: Gemo
Thank you for your question. Yes, you can use a conventional anti-mouse IgG secondary antibody for detection of this primary antibody. For more specific results, we do recommend using an isotype-specific secondary antibody (anti-mouse IgG2b), or our mouse IgGκ binding protein, e.g., m-IgGκ BP-HRP: sc-516102.
Answered by: Technical Support
Date published: 2020-03-09

Cantidad de anticuerpo FOXM1(G5) X TransCruz sc-376471 recomendado para usar en ChIP.

Asked by: femili12
Gracias por sus preguntas. Recomendamos el uso de 100-500 μg de sobrenadante y 0,1-1 μl de reactivo TransCruz (0,2-2 μg). Para obtener más información, haga clic en este enlace de nuestro protocolo ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your questions. We recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg). For further information, please click on this link to our ChIP protocol: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Technical Support
Date published: 2018-12-31

Which type of FOXM1  give good results for IHC on parrafin bock,human tissue breast cancer

Asked by: Madyan
Thank you for your question. We would recommend using our mouse monoclonal antibody, FOXM1 (G-5): sc-376471. We have several pieces of IHC(P) data in human tissue using this antibody on our website.
Answered by: Technical Support
Date published: 2017-08-22

Does the FOXM1 Antibody (G-5): sc-376471 detect isoform “C” of the FOXM1 target?

Asked by: Trav11
Thank you for your question. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-16

Does FOXM1 Antibody (G-5): sc-376471 react with Zebrafish samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 5 out of 5 by from 功能不错，敏感度很高，很好跑半年前试用了一周，感觉效果很棒，再次购买发现没让人失望，效果真的很不错，以后还会再买他家的抗体，都可以试试。
Date published: 2023-10-11
Rated 4 out of 5 by from 经理BACKGROUND The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The gene encoding human FOXM1 maps to chromosome 12p13.33. The transcription element that restricts FOXM1 expression to prolif- erating cells is located 300 bp upstream of the start codon. FOXM1 is most abundant in thymus, testis, small intestine and colon. Alternative splicing generates FOXM1A and FOXM1B isoforms that contain PEST regions involved in rapid protein degradation. A decrease in FOXM1 expression is associated with age-related defects in cellular proliferation. Conversely, an increase in FOXM1B expression in the livers of older transgenic mice restore hepatocyte DNA replication rates to the higher rate present in young livers. FOXM1B activates the transcription of cyclin B1, cyclin D1 and Cdc25B
Date published: 2021-06-23
Rated 5 out of 5 by from the greatest Ab i ever had!it works very well in both KD and OE systems i set up. believe me, commercial ABs of FOXM1 from other companies dont work at all. this showed clear band, at 1:250 dilution with senescent dermal fibroblast cell samples.
Date published: 2020-07-22
Rated 1 out of 5 by from Not specific for FOXM1 - Do not use this antibody!As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but
Date published: 2018-07-18
Rated 4 out of 5 by from Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 1 out of 5 by from Not sure if observed band is FOXM1Like the previous comments below, our group has also confirmed that this antibody may not be recognizing FOXM1. We observe a strong clear band at around 125-130 kDa with this antibody in our cell lines. However, upon using both siRNA and shRNA approaches with 3 different species, we could not observe a clear knockdown of FOXM1 with this antibody. In contrast, FOXM1 (A-11) sc-271746 showed knockdown of FOXM1 protein. Additionally, while this antibody can recognize overexpressed FOXM1, the exogenous FOXM1 appears as a second at around 110 kDa. The FOXM1 (A-11) antibody only recognizes a single band with much greater intensity. The FOXM1 (A-11) antibody also gave consistent results with CS #D12D5 and an Abcam antibody.
Date published: 2018-05-11
Rated 3 out of 5 by from Gives good band, but uncertain about specificityThe mouse G-5 antibody appears to detect primarily nuclear FOXM1; whereas the SC-500 rabbit antibody detects mainly cytoplasmic (and to some extent nuclear). The expression pattern in western blots, ICC, and IHC have confirmed this (images attached). Possibly due to the G-5 antibody unable to detect FOXM1 in cytoplasm if dimerized? It seems to detect FOXM1 differently in regards to localization; however, the MW appears to be correct. I still haven't quite figured this one out. In any case, I used the antibody at a 1:1000 dilution and secondary at 1:2500. I've noticed most SC antibodies work well at 1:250/500 primary and secondary at 1:2500. It has worked well with overexpression (human FOXM1b isoform) as well as siRNA knockdown in rat cells.
Date published: 2018-03-26
Rated 1 out of 5 by from Not sure the band we detect is FOXM1We have observed similar results than the one showed by elenaenzo with two different siRNA against FOXM1 in HeLa cells (an antibody against FOXM1 from another firm was used as a positive control on the same lysates).
Date published: 2018-03-16
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FOXM1 항체 (G-5) is rated 4.0 out of 5 by 28.
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