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Fibronectin Antibody (IST-9) - Western Blotting - Image 380652
피브로넥틴 항체(IST-9): sc-59826. Caki-1 (A), XP12RO (B) 및 A-10 (C) 전체 세포 용해물에서 피브로넥틴 발현의 웨스턴 블롯 분석.
피브로넥틴 항체(IST-9): sc-59826. Caki-1 (A), XP12RO (B) 및 A-10 (C) 전체 세포 용해물에서 피브로넥틴 발현의 웨스턴 블롯 분석.

Fibronectin 항체 (IST-9): sc-59826

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데이터 시트
  • Fibronectin 항체(IST-9) 는 마우스 monoclonal IgG1κ, 81간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human의 cellular Fibronectin에 해당하는 ED-A 도메인의 영역에 대해 제기됨
  • 안티-Fibronectin 항체 (IST-9)는 WB, IP, IF and IHC(P)으로 mouse, rat, human, porcine and canine유래의 cellular Fibronectin 를 감지하는 데에 추천한다.
  • 항-Fibronectin 항체(IST-9)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Fibronectin (IST-9): sc-59826의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 와 m-IgG1 BP-HRP 는 Fibronectin 항체 (IST-9) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Fibronectin 항체(IST-9) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

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섬유소원단 항체(IST-9)는 마우스, 쥐, 인간, 돼지, 개 샘플에서 웨스턴 블롯(WB), 면역침전(IP), 면역형광(IF), 면역조직화학 등의 방법을 통해 섬유소원단을 검출하는 마우스 단일클론 IgG1 카파 경쇄 항체입니다. 항피브로넥틴 항체(IST-9)는 비접합 형태와 아가로스, 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합체 형태로 제공됩니다. 피브로넥틴은 세포 부착, 운동성, 상처 치유에 중요한 역할을 하는 중요한 세포외 기질 당단백질로, 조직 복구와 재생에 필수적인 역할을 합니다. 이 고분자량 이량체 단백질은 주로 세포 표면, 세포외액, 혈장에 위치하며, 피브로넥틴은 세포와 세포외 기질 사이의 상호작용을 촉진합니다. 섬유소질의 대체 스플라이싱과 글리코실화 작용은 다양한 이소폼의 생성을 유도하는데, 그 중 일부는 특정 조직이나 악성 종양과 같은 병리학적 상태와 관련이 있습니다. 글리코실화 섬유소질과 섬유소질 스플라이스 변이체의 존재는 듀피트렌병에서 근섬유아세포 표현형의 형성과 관련이 있으며, 질병 맥락에서 번역 후 변형에 대한 이해의 중요성을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Fibronectin 항체 (IST-9)

sc-59826
200 µg/ml
$316.00

Fibronectin (IST-9): m-IgG Fc BP-HRP 번들

sc-526943
200 µg Ab; 10 µg BP
$354.00

Fibronectin (IST-9): m-IgG1 BP-HRP 번들

sc-532316
200 µg Ab; 20 µg BP
$354.00

Fibronectin 항체 (IST-9) AC

sc-59826 AC
500 µg/ml, 25% agarose
$416.00

Fibronectin 항체 (IST-9) HRP

sc-59826 HRP
200 µg/ml
$316.00

Fibronectin 항체 (IST-9) FITC

sc-59826 FITC
200 µg/ml
$330.00

Fibronectin 항체 (IST-9) PE

sc-59826 PE
200 µg/ml
$343.00

Fibronectin 항체 (IST-9) Alexa Fluor® 488

sc-59826 AF488
200 µg/ml
$357.00

Fibronectin 항체 (IST-9) Alexa Fluor® 546

sc-59826 AF546
200 µg/ml
$357.00

Fibronectin 항체 (IST-9) Alexa Fluor® 594

sc-59826 AF594
200 µg/ml
$357.00

Fibronectin 항체 (IST-9) Alexa Fluor® 647

sc-59826 AF647
200 µg/ml
$357.00

Fibronectin 항체 (IST-9) Alexa Fluor® 680

sc-59826 AF680
200 µg/ml
$357.00

Fibronectin 항체 (IST-9) Alexa Fluor® 790

sc-59826 AF790
200 µg/ml
$357.00

For IST-9 there is a picture of kidney tissue stained for IHC using IST-9 antibody. Can you please give me the reference for the image as i am looking for the IHC protocol used to produce this image i.e. processing and antigen optimisation process used.

Asked by: Pvirdee
Thanks for your question. The image is the results of in-house validation by Immunoperoxidase staining of formalin fixed, paraffin-embedded. You can find our protocol here https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining . Please contact technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2022-11-09

Hi Can I get a free sample of this antibody. (Fibronectin Antibody (IST-9): sc-59826) I wanted to see if it works with human sample for western blot. thank you Sindhu Saraswathy PhD Scientist Doheny Eye Institute

Asked by: Sindhu Saraswathy
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Does this antibody work for IF of formalin-fixed paraffin-embedded tissue?

Asked by: PhD Student
Thank you for your question. Yes, this antibody is recommended for IF and IHC(P) and will work perfectly fine for IF on formalin fixed tissues. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-10

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. For IHC, we recommend using a directly conjugated mouse monoclonal primary antibody. We recommend using SC-8422 for Fibronectin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 2 out of 5 by from no bandI used this antibody at 1:200 - 1:1000 as 1st antibody with 2nd mouse antibody at 1:1000 - 1:5000. Both 1st and 2nd antibody I dilute with 5% skim milk but still doesn't work. even on semi-dry or wet transfer also showed no band. Any kind suggestion please?
Date published: 2024-03-25
Rated 4 out of 5 by from Great productIt is good antibodies in WB. It works in dilution 1:1000
Date published: 2018-07-14
Rated 2 out of 5 by from Strong background in IF on rat frozen liverRat liver frozen sections were subjected to immunofluorescence using Fibronectin (IST-9) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Some signals could be observed in portal tracts, but background was strong.
Date published: 2018-03-14
Rated 4 out of 5 by from Works wellI received free samples and tried on western blotting. Tha bands are thick with no secondary bands. Need to dilute antibody concentrations to get good data.
Date published: 2018-02-12
Rated 5 out of 5 by from So nice!I received this Ab in mid-November 2017 and was very pleased to see it worked nicely! Will definitely purchase it...
Date published: 2018-01-13
Rated 3 out of 5 by from Staining of distal colon for fibronectin EDAI've had the chance to test the antibody for the staining of fibronectin EDA in the distal colon of mice that were developing fibrosis in the colon. Unfortunately, we did not see staining of the paraffin embedded slides, which also means that there is no background staining and the antibody is very specific. We are currently still trying to change some aspects of the staining protocol to check if we can get better staining.
Date published: 2017-12-04
Rated 5 out of 5 by from clear bandThis is a great antibody, the band was clear and significant.
Date published: 2017-05-13
Rated 5 out of 5 by from The band is clearlyWe detected positive signal in WB using mouse lung, strong signal;
Date published: 2017-04-22
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Fibronectin 항체 (IST-9) is rated 4.2 out of 5 by 13.
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